{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1300507200},"Data_type":{"Still_image":false,"Z_stack":false,"Video":true,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Mp4","File_path":"15788_web.mp4","Size":740192,"Mime_type":"video\/mp4"},{"File_type":"Zip","File_path":"15788.zip","Size":6860928,"Mime_type":"application\/zip"},{"File_type":"Jpeg","File_path":"15788.jpg","Size":47128,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"PUBLISHED":["J Cell Biol. 2009 Dec 14;187(6):831-45."],"Contributors":["George von Dassow","Koen J.C. Verbrugghe","Ann L. Miller","Jenny R. Sider","William M. Bement"],"PUBMED":["20008563"]},"BIOLOGICALPROCESS":[{"onto_name":"mitotic metaphase","onto_id":"GO:0000089"},{"onto_name":"mitotic anaphase","onto_id":"GO:0000090"},{"onto_name":"cytokinesis after mitosis","onto_id":"GO:0000281"},{"free_text":"trichostatin A treatment"}],"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"CELLLINE":{"onto_name":"Embryo","onto_id":"FMA:69068"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"16-cell purple urchin embryo expressing three tandem GFPs fused to the ensconsin microtubule-binding domain (EMTB-3G; Faire et al., 1999). The embryo was treated with 20 µM trichostatin A (TSA), an inhibitor of HDAC6 (a tubulin deacetylase) that selectively disrupts dynamic microtubules in mammalian cells, at time 00:00.  Within minutes of TSA addition in metaphase, asters were reduced to nearly nothing (04:00). Despite reduction in spindle length, cells enter anaphase (07:20) and complete cytokinesis (11:20\u201316:10). No microtubules connecting the mitotic apparatus to the cortex are visible.  Embryos were imaged with a laser scanning confocal microscope (Radiance 2000; Bio-Rad Laboratories) mounted on a compound microscope (E800; Nikon) using a 40× 1.3 NA Plan-Fluor oil lens at room temperature.  Corresponds to Figure 4A and first segment of video 4 in J Cell Biol. 2009 Dec 14;187(6):831-45."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"microtubule","onto_id":"GO:0005874"},{"free_text":"ensconsin"},{"onto_name":"astral microtubule","onto_id":"GO:0000235"},{"onto_name":"spindle","onto_id":"GO:0005819"}],"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},"GROUP_ID":"6184","TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"single-spot confocal microscopy","onto_id":"FBbi:00000252"},"DIMENSION":[{"Space":{"Image_size":512,"Pixel_size":{"unit":"microns","value":0.21},"axis":"X"}},{"Space":{"Image_size":512,"Pixel_size":{"unit":"microns","value":0.21},"axis":"Y"}},{"Time":{"unit":"seconds","value":10,"frame":"134"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Strongylocentrotus purpuratus","onto_id":"NCBITaxon:7668"},"PREPARATION":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"VISUALIZATIONMETHODS":{"onto_name":"EGFP","onto_id":"FBbi:00000082"}}},"Citation":{"Title":"George von Dassow, Koen J.C. Verbrugghe, Ann L. Miller, Jenny R. Sider, William M. Bement (2011) CIL:15788, Strongylocentrotus purpuratus. CIL. Dataset","ARK":"ark:\/b7295\/w9cil15788","DOI":"doi:10.7295\/W9CIL15788"}}}