Allen, K.D.; Duysen, M.E.; Staehelin, L.A. (2021). CIL:39077, Triticum sp., leaf. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J06H4G17

Wheat seeds were germinated and grown for 6 days in the dark, greened at high light intensity for 4 days, then prepared for thin section electron microscopy. This chloroplast from a wild-type plant shows the characteristic grana stacks of thylakoids (GT) and unstacked stromal regions (ST). Also seen are osmiophilic globules (PG). This set of grouped images, which also shows freeze-fracture views, includes the chlorophyll b-deficient CD3 mutant.

• 2021

• Allen, K.D.
• Duysen, M.E.
• Staehelin, L.A.

Leaf segments 1 cm from the tip were fixed with 2% glutaraldehyde, postfixed with 1% osmium tetroxide, dehydrated, and embedded in Spurrs resin. Thin sections were stained with uranly acetate and lead citrate and examined in a JEOL 100C transmission EM at 60 KV, and images recorded on film. See Fig 5 in K D Allen et al. 1988 Biogenesis of thylakoid membranes is controlled by light intensity in the conditional chlorophyll b-deficient CD3 mutant of wheat. J Cell Biol 107:907-919

Preparation: glutaraldehyde fixed tissue; tissue in plastic embedment
Relation to intact cell: microtome-sectioned tissue
Item type: film
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt; lead salt; osmium tetroxide
Data qualification: Processed

• Cell Image Library Group ID: 11355

• Triticum sp.

• Chloroplast
• Granum
• Leaf
• Plastid thylakoid membrane

• Organelle organization
• Photosynthesis

• data
• image

• No linguistic content; Not applicable

Samplenumber: 39077

Doi: https://doi.org/10.6075/J06H4G17

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL39077

Reference

• Allen KD, Duysen ME, Staehelin LA. Biogenesis of thylakoid membranes is controlled by light intensity in the conditional chlorophyll b-deficient CD3 mutant of wheat. J Cell Biol. 1988 Sep;107(3):907-19. https://doi.org/10.1083/jcb.107.3.907
• K D Allen, ME Duysen, and LA Stahelin 1988 Biogenesis of thylakoid membranes is controlled by light intensity in the conditional chlorophyll b-deficient CD3 mutant of wheat. J Cell Biol 107:907-919 https://www.ncbi.nlm.nih.gov/pubmed/?term=3047153

Other resource

• Wellcome Images: http://images.wellcome.ac.uk/

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2022-08-12