{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1321851600},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"39077.tif","Size":1800000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"39077.jpg","Size":334892,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"39077.zip","Size":1761612,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Label":"Journal of Cell Biology","Href":"http:\/\/jcb.rupress.org\/content\/107\/3\/907.long"}],"PUBLISHED":["K D Allen, ME Duysen, and LA Stahelin  1988 Biogenesis of thylakoid membranes is controlled by light intensity in the conditional chlorophyll b-deficient CD3 mutant of wheat. J Cell Biol 107:907-919"],"Contributors":["K D Allen","M E Duysen","L A Staehelin"],"PUBMED":["3047153"]},"BIOLOGICALPROCESS":[{"onto_name":"photosynthesis","onto_id":"GO:0015979"},{"onto_name":"organelle organization","onto_id":"GO:0006996"}],"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"Wheat seeds were germinated and grown for 6 days in the dark, greened at high light intensity for 4 days, then prepared for thin section electron microscopy. This chloroplast from a wild-type plant shows the characteristic grana stacks of thylakoids (GT) and unstacked stromal regions (ST). Also seen are osmiophilic globules (PG). This set of grouped images, which also shows freeze-fracture views, includes the chlorophyll b-deficient CD3 mutant."},"ITEMTYPE":{"onto_name":"film","onto_id":"FBbi:00000303"},"CELLULARCOMPONENT":[{"onto_name":"chloroplast","onto_id":"GO:0009507"},{"onto_name":"plastid thylakoid membrane","onto_id":"GO:0055035"},{"onto_name":"granum","onto_id":"GO:0009542"}],"SOURCEOFCONTRAST":{"onto_name":"differences in adsorption or binding of stain","onto_id":"FBbi:00000598"},"RELATIONTOINTACTCELL":{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"},"GROUP_ID":"11355","TECHNICALDETAILS":{"free_text":"Leaf segments 1 cm from the tip were fixed with 2% glutaraldehyde, postfixed with 1% osmium tetroxide, dehydrated, and embedded in Spurrs resin. Thin sections were stained with uranly acetate and lead citrate and examined in a JEOL 100C transmission EM at 60 KV, and images recorded on film.  See Fig 5 in K D Allen et al. 1988 Biogenesis of thylakoid membranes is controlled by light intensity in the conditional chlorophyll b-deficient CD3 mutant of wheat. J Cell Biol 107:907-919"},"DATAQUALIFICATION":{"free_text":"PROCESSED"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},"DIMENSION":[{"Space":{"Image_size":1812,"axis":"X"}},{"Space":{"Image_size":972,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Triticum sp.","onto_id":"NCBITaxon:4569"},"PREPARATION":[{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},{"onto_name":"tissue in plastic embedment","onto_id":"FBbi:00000016"}],"VISUALIZATIONMETHODS":[{"onto_name":"uranyl salt","onto_id":"FBbi:00000569"},{"onto_name":"lead salt","onto_id":"FBbi:00000570"},{"onto_name":"osmium tetroxide","onto_id":"FBbi:00000571"}],"CELLTYPE":{"free_text":"leaf"}}},"Citation":{"Title":"K D Allen, M E Duysen, L A Staehelin (2011) CIL:39077, Triticum sp., leaf. CIL. Dataset","ARK":"ark:\/b7295\/w9cil39077","DOI":"doi:10.7295\/W9CIL39077"}}}