CIL:25112, Mus musculus, fibroblast
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- Collection
- Cite This Work
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Politz, Joan (2021). CIL:25112, Mus musculus, fibroblast. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J09Z94X2
- Description
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Mouse 3T3 fibroblast shown in phase contrast (left) and immuno-stained for the nucleolar protein nucleostemin (right, green).
- Date Issued
- 2021
- Researcher
- Methods
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Data were collected using a Leica DMIRB microscope equipped with a 100x objective (N.A. 1.4) and appropriate filter sets, and images captured using a Quantix 57 CCD camera (Roper Scientific Photometrics). For high resolution spatial mapping, three-dimensional optical stacks (containing 21 consecutive 0.25 micron slices) were captured using a PIFOC microscope focusing drive (Polytec PI). Images were dark current subtracted and intensity scaled See Fig 1 CD in Politz et al., 2005 Mol Biol Cell 16:3401-3410.
- Technical Details
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Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: phase contrast microscopy; fluorescence microscopy
Parameter imaged: elastic scattering of photons; fluorescence emission
Source of contrast: differences in amount of elastic light scattering; distribution of epitope
Visualization methods: Fluorescein (FITC)
Processing history: multipanel montage
Data qualification: Processed - Series
- Scientific Name
- Anatomy
- Topics
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- Language
- No linguistic content; Not applicable
- Identifier
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Samplenumber: 25112
- Related Resources
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL25112
- Joan C. Ritland Politz, Ilvin Polena, Ian Trask, David P. Bazett-Jones, and Thoru Pederson. A Nonribosomal Landscape in the Nucleolus Revealed by the Stem Cell Protein Nucleostemin. Molecular Biology of the Cell 2005 16:7, 3401-3410. https://doi.org/10.1091/mbc.e05-02-0106
- Politz et al., 2005 Mol Biol Cell 16:3401-3410 https://www.ncbi.nlm.nih.gov/pubmed/?term=15857956
Source data
Reference
- License
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Creative Commons Attribution 4.0 International Public License
- Rights Holder
- UC Regents
- Copyright
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Under copyright (US)
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12