{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1301544000},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"25112.tif","Size":400000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"25112.jpg","Size":21860,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"25112.zip","Size":304770,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"OTHER":["Ilvin Polena","Ian Trask","David Bazett-Jones","Thoru Pederson"],"URLs":[{"Label":"Molecular Biology of the Cell","Href":"http:\/\/www.molbiolcell.org\/content\/16\/7\/3401.long"}],"PUBLISHED":["Politz et al., 2005 Mol Biol Cell 16:3401-3410"],"Contributors":["Joan Politz"],"PUBMED":["15857956"]},"BIOLOGICALPROCESS":[{"onto_name":"ribosome biogenesis","onto_id":"GO:0042254"},{"onto_name":"nucleus organization","onto_id":"GO:0006997"},{"onto_name":"RNA metabolic process","onto_id":"GO:0016070"},{"onto_name":"nucleolus organization","onto_id":"GO:0007000"}],"PROCESSINGHISTORY":{"free_text":"multipanel montage"},"CELLLINE":{"free_text":"3T3"},"PARAMETERIMAGED":[{"onto_name":"elastic scattering of photons","onto_id":"FBbi:00000587"},{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"}],"IMAGEDESCRIPTION":{"free_text":"Mouse 3T3 fibroblast shown in phase contrast (left) and immuno-stained for the nucleolar protein nucleostemin (right, green)."},"ITEMTYPE":{"onto_name":"charge coupled device (CCD)","onto_id":"FBbi:00000294"},"CELLULARCOMPONENT":[{"onto_name":"nucleolus","onto_id":"GO:0005730"},{"onto_name":"nucleus","onto_id":"GO:0005634"}],"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":[{"onto_name":"differences in amount of elastic light scattering","onto_id":"FBbi:00000600"},{"onto_name":"distribution of epitope","onto_id":"FBbi:00000592"}],"GROUP_ID":"11204","TECHNICALDETAILS":{"free_text":"Data were collected using a Leica DMIRB microscope equipped with a 100x objective (N.A. 1.4) and appropriate filter sets, and images captured using a Quantix 57 CCD camera (Roper Scientific Photometrics). For high resolution spatial mapping, three-dimensional optical stacks (containing 21 consecutive 0.25 micron slices) were captured using a PIFOC microscope focusing drive (Polytec PI). Images were dark current subtracted and intensity scaled See Fig 1 CD in Politz et al., 2005 Mol Biol Cell 16:3401-3410."},"DATAQUALIFICATION":{"free_text":"PROCESSED"},"TERMSANDCONDITIONS":{"free_text":"attribution_cc_by"},"IMAGINGMODE":[{"onto_name":"phase contrast microscopy","onto_id":"FBbi:00000247"},{"onto_name":"fluorescence microscopy","onto_id":"FBbi:00000246"}],"DIMENSION":[{"Space":{"Image_size":453,"axis":"X"}},{"Space":{"Image_size":224,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Mus musculus","onto_id":"NCBITaxon:10090"},"PREPARATION":{"onto_name":"formaldehyde fixed tissue","onto_id":"FBbi:00000010"},"VISUALIZATIONMETHODS":{"onto_name":"Fluorescein (FITC)","onto_id":"FBbi:00000451"},"CELLTYPE":{"onto_name":"fibroblast","onto_id":"CL:0000057"}}},"Citation":{"Title":"Joan Politz (2011) CIL:25112, Mus musculus, fibroblast. CIL. Dataset","ARK":"ark:\/b7295\/w9cil25112","DOI":"doi:10.7295\/W9CIL25112"}}}