Fischer, Robert S.; Waterman, Clare M. (2021). CIL:7330, Mus musculus, blood vessel endothelial cell, primary cell line cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0445KJ5

Primary aortic endothelial cells from transgenic mouse embedded in 3D collagen gels. Cell was fixed with 3% paraformaldehyde and stained with mouse antibody to phosphomyosin regulatory light chain (red), followed by anti-mouse Alexa 561 goat antibody and Alexa 488 phalloidin to stain for F-actin (green).
Size and scale: 0.0789 micron/pixel, 1352x1040 pixels
Images collected with a spinning disk confocal on a Nikon TE1000 with a 60X 1.2NA water objective using 300 ms exposure for the red channel and 200 ms for the green channel.
See Fischer et al for details regarding preparation. http://www.ncbi.nlm.nih.gov/pubmed/19185493

• 2021

• Fischer, Robert S.
• Waterman, Clare M.

Preparation: formaldehyde fixed tissue
Relation to intact cell: dispersed cells in vitro; cells in 3D matrix
Item type: recorded image
Imaging mode: spinning disk confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain
Visualization methods: Alexa Fluor 488; Alexa Fluor 568; primary antibody plus labeled secondary antibody; label conjugated to probe
Processing history: formaldehyde fixed tissue

• Mus musculus

• Actin cytoskeleton
• Blood vessel endothelial cell
• Myosin complex
• Primary cell line cell

• Actomyosin structure organization
• Myosin phosphatase activity

• data
• image

• No linguistic content; Not applicable

Samplenumber: 7330

Doi: https://doi.org/10.6075/J0445KJ5

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL7330

Reference

• PubMed article: https://www.ncbi.nlm.nih.gov/pubmed/?term=19185493

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2022-08-12