{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1291525200},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"7330.zip","Size":4353580,"Mime_type":"application\/zip"},{"File_type":"Jpeg","File_path":"7330.jpg","Size":133763,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"OME_tif","File_path":"7330.tif","Size":4400000,"Mime_type":"image\/tif"}],"CORE":{"ATTRIBUTION":{"OTHER":["NHLBI\/NIH"],"Contributors":["Robert S. Fischer","Clare M. Waterman"],"PUBMED":["19185493"]},"BIOLOGICALPROCESS":{"onto_name":"actomyosin structure organization","onto_id":"GO:0031032"},"PROCESSINGHISTORY":{"onto_name":"formaldehyde fixed tissue","onto_id":"FBbi:00000010"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Primary aortic endothelial cells from transgenic mouse embedded in 3D collagen gels. Cell was fixed with 3% paraformaldehyde and stained with mouse antibody to phosphomyosin regulatory light chain (red), followed by anti-mouse Alexa 561 goat antibody and Alexa 488 phalloidin to stain for F-actin (green).  \nSize and scale: 0.0789 micron\/pixel, 1352x1040 pixels \nImages collected with a spinning disk confocal on a Nikon TE1000 with a 60X 1.2NA water objective using 300 ms exposure for the red channel and 200 ms for the green channel.\nSee Fischer et al for details regarding preparation. http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/19185493"},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"actin cytoskeleton","onto_id":"GO:0015629"},{"onto_name":"myosin complex","onto_id":"GO:0016459"}],"RELATIONTOINTACTCELL":[{"onto_name":"dispersed cells in vitro","onto_id":"FBbi:00000611"},{"free_text":"cells in 3D matrix"}],"SOURCEOFCONTRAST":{"onto_name":"differences in adsorption or binding of stain","onto_id":"FBbi:00000598"},"MOLECULARFUNCTION":{"onto_name":"myosin phosphatase activity","onto_id":"GO:0017018"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"spinning disk confocal microscopy","onto_id":"FBbi:00000253"},"DIMENSION":[{"Space":{"Image_size":1352,"Pixel_size":{"unit":"microns","value":0.789},"axis":"X"}},{"Space":{"Image_size":1040,"Pixel_size":{"unit":"microns","value":0.789},"axis":"Y"}},{"Wavelength":{"unit":"nanometers","value":"488,561"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Mus musculus","onto_id":"NCBITaxon:10090"},"PREPARATION":{"onto_name":"formaldehyde fixed tissue","onto_id":"FBbi:00000010"},"VISUALIZATIONMETHODS":[{"onto_name":"Alexa Fluor 488","onto_id":"FBbi:00000440"},{"onto_name":"Alexa Fluor 568","onto_id":"FBbi:00000442"},{"onto_name":"primary antibody plus labeled secondary antibody","onto_id":"FBbi:00000156"},{"onto_name":"label conjugated to probe","onto_id":"FBbi:00000128"}],"CELLTYPE":[{"onto_name":"blood vessel endothelial cell","onto_id":"CL:0000071"},{"onto_name":"primary cell line cell","onto_id":"CL:0000001"}]}},"Citation":{"Title":"Robert S. Fischer, Clare M. Waterman (2010) CIL:7330, Mus musculus, blood vessel endothelial cell, primary cell line cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil7330","DOI":"doi:10.7295\/W9CIL7330"}}}