Howard, Louisa (2021). CIL:39785, Lytechinus pictus, mesenchymal cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J04F1Q24

Scanning electron microscope image of Strongylocentrotus drobachiensus [sea urchin] embryo at the late gastrula stage. Embryo was cut to reveal blastocoel cavity. The fertilization envelope dissolves during swimming blastula stage; therefore, the cilia and microvilli can be seen on the outer epithelial layer. The primary mesenchyme cells have formed a syncytial ring, which is the precursor to spicule formation(developing calcareous larval skeleton). A portion of the archenteron can also be seen. A high magnification view of a region of this image is available as CIL:39789.This image is part of a group of SEM images of sea urchin embryos at various stages of development. The group is available as CIL:39782-39791.

• 2021

• Howard, Louisa

Images taken on a JEOL 35C SEM.
Processing protocol:
1. 2% GTA in 75% MBL SW or FilteredSW
FSW - fix for 1.5hrs. RT.
2. Rinse with 0.1M NaCacodylate buffer pH 7.4
3. 0.5% OSO4 in .075M NaCacodylate buffer pH 7.4 - 30' in the cold
4. Rinse in 0.1M NaCacodylate buffer ph 7.4
5. Dehydrate thru ETOH: 30%, 50%, 70%, 95% for 5 min each
6. 100% - 3 changes - 15' each
7. Critical Point Dry
8. Stick embryos down onto silver paint. Gently crack open with glass needle (pipette drawn out to a fine point).
9. Sputter coat with AuPd.

Preparation: glutaraldehyde fixed tissue; critical_point dried specimen; ethanol dehydration
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: scanning electron microscopy (SEM)
Parameter imaged: elastic scattering of electrons
Source of contrast: differences in deposition of metal shadow
Visualization methods: gold; visualization of contiguous regions; osmium tetroxide
Processing history: unprocessed raw data

• Cell Image Library Group ID: 10724

• Lytechinus pictus

• Cell surface
• Cilium
• Mesenchymal cell
• Microvillus

• Embryonic morphogenesis

• data
• image

• No linguistic content; Not applicable

Samplenumber: 39785

Doi: https://doi.org/10.6075/J04F1Q24

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL39785

Other resource

• Dartmouth College Electron Microscope Facility (search on the string "sea urchin"): https://www.dartmouth.edu/emlab/gallery/index.php

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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12