{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1329195600},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"39785.tif","Size":1000000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"39785.jpg","Size":88781,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"39785.zip","Size":93742,"Mime_type":"application\/zip"},{"File_type":"Jpeg","File_path":"39785_orig.jpg","Size":93604,"Mime_type":"image\/jpeg"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Label":"Dartmouth EM Facility","Href":"http:\/\/remf.dartmouth.edu\/images\/SeaUrchinSEM\/source\/4.html"}],"Contributors":["Louisa Howard"]},"BIOLOGICALPROCESS":{"onto_name":"embryonic morphogenesis","onto_id":"GO:0048598"},"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"PARAMETERIMAGED":{"onto_name":"elastic scattering of electrons","onto_id":"FBbi:00000586"},"IMAGEDESCRIPTION":{"free_text":"Scanning electron microscope image of Strongylocentrotus drobachiensus [sea urchin] embryo at the late gastrula stage. Embryo was cut to reveal blastocoel cavity. The fertilization envelope dissolves during swimming blastula stage; therefore, the cilia and microvilli can be seen on the outer epithelial layer. The primary mesenchyme cells have formed a syncytial ring, which is the precursor to spicule formation(developing calcareous larval skeleton). A portion of the archenteron can also be seen.  A high magnification view of a region of this image is available as CIL:39789.  This image is part of a group of SEM images of sea urchin embryos at various stages of development.  The group is available as CIL:39782-39791."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"cilium","onto_id":"GO:0005929"},{"onto_name":"microvillus","onto_id":"GO:0005902"},{"onto_name":"cell surface","onto_id":"GO:0009986"}],"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":{"onto_name":"differences in deposition of metal shadow","onto_id":"FBbi:00000601"},"GROUP_ID":"10724","TECHNICALDETAILS":{"free_text":"Images taken on a  JEOL 35C SEM.  \nProcessing protocol:\n1. 2% GTA in 75% MBL SW or FilteredSW \nFSW - fix for 1.5hrs. RT.\n2. Rinse with 0.1M NaCacodylate buffer pH 7.4\n3. 0.5% OSO4 in .075M NaCacodylate buffer pH 7.4 - 30' in the cold\n4. Rinse in 0.1M NaCacodylate buffer ph 7.4\n5. Dehydrate thru ETOH: 30%, 50%, 70%, 95% for 5 min each\n6. 100% - 3 changes - 15' each\n7. Critical Point Dry\n8. Stick embryos down onto silver paint. Gently crack open with glass needle (pipette drawn out to a fine point).\n9. Sputter coat with AuPd."},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"scanning electron microscopy (SEM)","onto_id":"FBbi:00000257"},"DIMENSION":[{"Space":{"Image_size":640,"axis":"X"}},{"Space":{"Image_size":480,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Lytechinus pictus","onto_id":"NCBITaxon:7653"},"PREPARATION":[{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},{"free_text":"ethanol dehydration"},{"onto_name":"critical_point dried specimen","onto_id":"FBbi:00000581"}],"VISUALIZATIONMETHODS":[{"onto_name":"gold","onto_id":"FBbi:00000202"},{"onto_name":"visualization of contiguous regions","onto_id":"FBbi:00000396"},{"onto_name":"osmium tetroxide","onto_id":"FBbi:00000571"}],"CELLTYPE":{"onto_name":"mesenchymal cell","onto_id":"CL:0000134"}}},"Citation":{"Title":"Louisa Howard (2012) CIL:39785, Lytechinus pictus, mesenchymal cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil39785","DOI":"doi:10.7295\/W9CIL39785"}}}