Maresca, Thomas; Salmon, Edward (2021). CIL:25307, Drosophila melanogaster. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0G15ZRG

Movie showing kinetochore alignment during mitosis in Drosophila S2 cells expressing GFP-Ndc80 to label kinetochores. One pair of chromatids becomes detached from the spindle, and anaphase is delayed until the pair is recaptured.
Images were recorded at 60 sec intervals using a spinning disc confocal microscope on a Nikon TE-200 stand with a 100x 1.4 NA Plan APO DIC objective lens and recorded with a Hamamatsu Orca-ER CCD. See Maresca and Salmon, 2009 J Cell Biol 184:373-281.

• 2021

• Maresca, Thomas
• Salmon, Edward

Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: spinning disk confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain
Visualization methods: Green fluorescent proteins from Aequorea
Processing history: unprocessed raw data

• Drosophila melanogaster

• Spindle

• Mitosis
• S2

• data
• image
• video

• No linguistic content; Not applicable

Samplenumber: 25307

Doi: https://doi.org/10.6075/J0G15ZRG

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL25307

Reference

• Maresca and Salmon, 2009 J Cell Biol 184:373-281 https://www.ncbi.nlm.nih.gov/pubmed/?term=19193623

CC0 1.0 Universal Public Domain Dedication

Creative Commons Public Domain Dedication

Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.

Constraint(s) on Use: This work may be used without prior permission.

Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12