{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1303099200},"Data_type":{"Still_image":false,"Z_stack":false,"Video":true,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Jpeg","File_path":"25307.jpg","Size":23615,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"25307.zip","Size":7667840,"Mime_type":"application\/zip"},{"File_type":"Mp4","File_path":"25307_web.mp4","Size":274721,"Mime_type":"video\/mp4"}],"CORE":{"ATTRIBUTION":{"PUBLISHED":["Maresca and Salmon, 2009 J Cell Biol 184:373-281"],"Contributors":["Thomas Maresca","Edward Salmon"],"PUBMED":["19193623"]},"BIOLOGICALPROCESS":{"onto_name":"mitosis","onto_id":"GO:0007067"},"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"CELLLINE":{"free_text":"S2"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Movie showing kinetochore alignment during mitosis in Drosophila S2 cells expressing GFP-Ndc80 to label kinetochores. One pair of chromatids becomes detached from the spindle, and anaphase is delayed until the pair is recaptured. \nImages were recorded at 60 sec intervals using a spinning disc confocal microscope on a Nikon TE-200 stand with a 100x 1.4 NA Plan APO DIC objective lens and recorded with a Hamamatsu Orca-ER CCD. See Maresca and Salmon, 2009 J Cell Biol 184:373-281."},"ITEMTYPE":{"onto_name":"charge coupled device (CCD)","onto_id":"FBbi:00000294"},"CELLULARCOMPONENT":{"onto_name":"spindle","onto_id":"GO:0005819"},"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":{"onto_name":"differences in adsorption or binding of stain","onto_id":"FBbi:00000598"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"spinning disk confocal microscopy","onto_id":"FBbi:00000253"},"DIMENSION":[{"Space":{"Image_size":400,"Pixel_size":{"unit":"microns","value":0.065},"axis":"X"}},{"Space":{"Image_size":400,"Pixel_size":{"unit":"microns","value":0.065},"axis":"Y"}},{"Time":{"unit":"seconds","value":60,"frame":"44"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Drosophila melanogaster","onto_id":"NCBITaxon:7227"},"PREPARATION":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"VISUALIZATIONMETHODS":{"onto_name":"Green fluorescent proteins from Aequorea","onto_id":"FBbi:00000437"}}},"Citation":{"Title":"Thomas Maresca, Edward Salmon (2011) CIL:25307, Drosophila melanogaster. CIL. Dataset","ARK":"ark:\/b7295\/w9cil25307","DOI":"doi:10.7295\/W9CIL25307"}}}