Wheeler, Richard (2021). CIL:10602, Leishmania mexicana, Mus musculus, macrophage, parasite. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J00Z720K

Macrophage cell line cell infected with Leishmania mexicana. Five amastigotes can be seen within a single large parasitophorous vacuole in the macrophage. Raw 264.7 cells (a leukaemic mouse macrophage line) were infected with amastigote form Leishmania mexicana (WHO strain MNYC/BZ/62/M379) at an MOI of 10 and maintained in RPMI for 4 days. Cells were fixed with 2% paraformaldehyde, and DNA was labelled with propidium iodide following incubation with 50 ug/ml RNaseA for 30 min at room temperature. Z stack of 93 images with a z spacing of 0.194 um captured with a 100x/1.4 NA objective lens on a DM5500 B epifluorescence microscope (Leica) with an Orca cooled CCD camera (Hamamatsu). Note there is a phase channel image that is not currently displayed, but can be turned on in the full viewer mode.

• 2021

• Wheeler, Richard

Preparation: formaldehyde fixed tissue
Relation to intact cell: permeabilized tissue
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission; optical path length gradient
Source of contrast: differences in adsorption or binding of stain; boundaries between regions with different refractive index
Visualization methods: propidium iodide
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation

• Cell Image Library Group ID: 3667

• Leishmania mexicana
• Mus musculus

• Macrophage
• Parasite
• Parasite-containing vacuole

• Amastigote form Leishmania mexicana
• Parasite entry into host cell forming a parasitophorous vacuole
• RAW264.7
• WHO strain MNYC/BZ/62/M379

• data
• image

• No linguistic content; Not applicable

Samplenumber: 10602

Doi: https://doi.org/10.6075/J00Z720K

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL10602

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2022-08-12