{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1293080400},"Data_type":{"Still_image":false,"Z_stack":true,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"10602.zip","Size":171465525,"Mime_type":"application\/zip"},{"File_type":"Jpeg","File_path":"10602.jpg","Size":16556,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"OME_tif","File_path":"10602.tif","Size":171700000,"Mime_type":"image\/tif"}],"CORE":{"ATTRIBUTION":{"Contributors":["Richard Wheeler"]},"BIOLOGICALPROCESS":[{"free_text":"amastigote form Leishmania mexicana"},{"free_text":"parasite entry into host cell forming a parasitophorous vacuole"}],"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"CELLLINE":[{"free_text":"WHO strain MNYC\/BZ\/62\/M379"},{"onto_name":"RAW264.7","onto_id":"MCC:0000390"}],"PARAMETERIMAGED":[{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},{"onto_name":"optical path length gradient","onto_id":"FBbi:00000312"}],"IMAGEDESCRIPTION":{"free_text":"Macrophage cell line cell infected with Leishmania mexicana. Five amastigotes can be seen within a single large parasitophorous vacuole in the macrophage. Raw 264.7 cells (a leukaemic mouse macrophage line) were infected with amastigote form Leishmania mexicana (WHO strain MNYC\/BZ\/62\/M379) at an MOI of 10 and maintained in RPMI for 4 days. Cells were fixed with 2% paraformaldehyde, and DNA was labelled with propidium iodide following incubation with 50 ug\/ml RNaseA for 30 min at room temperature. Z stack of 93 images with a z spacing of 0.194 um captured with a 100x\/1.4 NA objective lens on a DM5500 B epifluorescence microscope (Leica) with an Orca cooled CCD camera (Hamamatsu).  Note there is a phase channel image that is not currently displayed, but can be turned on in the full viewer mode."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":{"free_text":"parasite-containing vacuole"},"RELATIONTOINTACTCELL":{"onto_name":"permeabilized tissue","onto_id":"FBbi:00000093"},"SOURCEOFCONTRAST":[{"onto_name":"differences in adsorption or binding of stain","onto_id":"FBbi:00000598"},{"onto_name":"boundaries between regions with different refractive index","onto_id":"FBbi:00000599"}],"GROUP_ID":"3667","DATAQUALIFICATION":{"free_text":"RAW;spatialmeasurements;intensitiesquantitation"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"fluorescence microscopy","onto_id":"FBbi:00000246"},"DIMENSION":[{"Space":{"Image_size":640,"Pixel_size":{"unit":"microns","value":0.0641},"axis":"X"}},{"Space":{"Image_size":480,"Pixel_size":{"unit":"microns","value":0.0641},"axis":"Y"}},{"Space":{"Image_size":93,"Pixel_size":{"unit":"nanometers","value":0.194},"axis":"Z"}},{"Wavelength":{"unit":"nanometers","value":"dic, fluorescence"}}],"NCBIORGANISMALCLASSIFICATION":[{"onto_name":"Leishmania mexicana","onto_id":"NCBITaxon:5665"},{"onto_name":"Mus musculus","onto_id":"NCBITaxon:10090"}],"PREPARATION":{"onto_name":"formaldehyde fixed tissue","onto_id":"FBbi:00000010"},"VISUALIZATIONMETHODS":{"onto_name":"propidium iodide","onto_id":"FBbi:00000047"},"CELLTYPE":[{"onto_name":"macrophage","onto_id":"CL:0000235"},{"free_text":"parasite"}]}},"Citation":{"Title":"Richard Wheeler (2010) CIL:10602, Leishmania mexicana, Mus musculus, macrophage, parasite. CIL. Dataset","ARK":"ark:\/b7295\/w9cil10602","DOI":"doi:10.7295\/W9CIL10602"}}}