Ammar, El-Desouky; Hogenhout, Saskia A.; Nault, L.R. (2021). CIL:25368, Maize mosaic virus, Peregrinus maidis, secretory cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0NS0SN9

Aggregates of Maize mosaic virus (MMV, Rhabdoviridae) in cells of the salivary gland of the insect vector Peregrinus maidis (planthopper, Hemiptera, Delphacidae). Antibodies to MMV identify aggregates (green/yellow fluorescence). In the salivary gland, MMV aggregates are found mainly in the cell periphery or intercellularly, in contrast to other tissues in the insect vector that accumulate mainly around the nuclei (red). Accompanying image in this group contains labels.

Methods: Dissected insect organs were fixed in paraformaldehyde, labeled with primary antibodies to MMV, and with secondary antibodies: Alexa Fluor-488 (green), and later stained with the nuclear stain propidium iodide (red). Whole mounts were examined with confocal laser- scanning microscope (Leica TCS SP).

Associated references:
Ammar, E.-D. and S. A. Hogenhout, S.A. (2008). A neurotropic route for Maize mosaic virus (Rhabdoviridae) in its planthopper vector Peregrinus maidis. Virus Research 131: 77-85.
Ammar, E.-D. and Nault, L.R. (1985). Assembly and accumulation sites of Maize mosaic virus in its planthopper vector. Intervirology 24: 33-41.

• 2021

• Ammar, El-Desouky
• Hogenhout, Saskia A.
• Nault, L.R.

Preparation: formaldehyde fixed tissue; permeabilized tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain; distribution of epitope
Visualization methods: primary antibody plus labeled secondary antibody; Alexa Fluor 488; propidium iodide
Processing history: recorded image
Data qualification: Raw;spatialmeasurements

• Cell Image Library Group ID: 8078

• Maize mosaic virus
• Peregrinus maidis

• Cell periphery
• Nucleus
• Secretory cell

• Viral transmission by vector

• data
• image

• No linguistic content; Not applicable

Samplenumber: 25368

Doi: https://doi.org/10.6075/J0NS0SN9

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL25368

Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License

• UC Regents

Under copyright (US)

Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.

Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.

Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12