{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1305172800},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Jpeg","File_path":"25368.jpg","Size":316470,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"25368.zip","Size":11560578,"Mime_type":"application\/zip"},{"File_type":"OME_tif","File_path":"25368.tif","Size":7700000,"Mime_type":"image\/tif"}],"CORE":{"ATTRIBUTION":{"Contributors":["El-Desouky Ammar","Saskia A. Hogenhout","L. R. Nault"]},"BIOLOGICALPROCESS":{"onto_name":"viral transmission by vector","onto_id":"GO:0044009"},"PROCESSINGHISTORY":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Aggregates of Maize mosaic virus (MMV, Rhabdoviridae) in cells of the salivary gland of the insect vector Peregrinus maidis (planthopper, Hemiptera, Delphacidae). Antibodies to MMV identify aggregates (green\/yellow fluorescence).  In the salivary gland, MMV aggregates are found mainly in the cell periphery or intercellularly, in contrast to other tissues in the insect vector that accumulate mainly around the nuclei (red).  Accompanying image in this group contains labels.\n\nMethods: Dissected insect organs were fixed in paraformaldehyde, labeled with primary antibodies to MMV, and with secondary antibodies: Alexa Fluor-488 (green), and later stained with the nuclear stain propidium iodide (red). Whole mounts were examined with confocal laser- scanning microscope (Leica TCS SP).\n\nAssociated references: \nAmmar, E.-D. and S. A. Hogenhout, S.A. (2008). A neurotropic route for Maize mosaic virus (Rhabdoviridae) in its planthopper vector Peregrinus maidis. Virus Research 131: 77-85.\nAmmar, E.-D. and Nault, L.R. (1985). Assembly and accumulation sites of Maize mosaic virus in its planthopper vector. Intervirology 24: 33-41."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"nucleus","onto_id":"GO:0005634"},{"onto_name":"cell periphery","onto_id":"GO:0071944"}],"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":[{"onto_name":"differences in adsorption or binding of stain","onto_id":"FBbi:00000598"},{"onto_name":"distribution of epitope","onto_id":"FBbi:00000592"}],"GROUP_ID":"8078","DATAQUALIFICATION":{"free_text":"RAW;spatialmeasurements"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"confocal microscopy","onto_id":"FBbi:00000251"},"DIMENSION":[{"Space":{"Image_size":1600,"Pixel_size":{"unit":"microns","value":0.678},"axis":"X"}},{"Space":{"Image_size":1600,"Pixel_size":{"unit":"microns","value":0.678},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":[{"onto_name":"Maize mosaic virus","onto_id":"NCBITaxon:279896"},{"onto_name":"Peregrinus maidis","onto_id":"NCBITaxon:222432"}],"PREPARATION":[{"onto_name":"formaldehyde fixed tissue","onto_id":"FBbi:00000010"},{"onto_name":"permeabilized tissue","onto_id":"FBbi:00000093"}],"VISUALIZATIONMETHODS":[{"onto_name":"primary antibody plus labeled secondary antibody","onto_id":"FBbi:00000156"},{"onto_name":"Alexa Fluor 488","onto_id":"FBbi:00000440"},{"onto_name":"propidium iodide","onto_id":"FBbi:00000047"}],"CELLTYPE":{"onto_name":"secretory cell","onto_id":"CL:0000151"}}},"Citation":{"Title":"El-Desouky Ammar, Saskia A. Hogenhout, L. R. Nault (2011) CIL:25368, Maize mosaic virus, Peregrinus maidis, secretory cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil25368","DOI":"doi:10.7295\/W9CIL25368"},"Microbial_type":["virus"]}}