Cazares, Victor; Chao, Luke; Galbraith, James A.; Galbraith, Catherine G. (2021). CIL:36147, Drosophila melanogaster, epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J02N50XM

The first image in this multi-image tiff file is a structured illumination image (SIM) of microtubules in a Drosophila S2 cell. The second image is the corresponding diffraction limited image obtained with conventional fluorescence microscopy. Note the two-fold increase in spatial resolution in the SIM image compared to the diffraction limited image. The z-series are available as CIL 36797. Images were collected as part of the Marine Biological Laboratory Neurobiology Course, Summer 2011.

• 2021

• Cazares, Victor
• Chao, Luke
• Galbraith, Catherine G.
• Galbraith, James A.

Cells were plated on Con-A coated coverslips, chemically fixed, and labeled with an anti-tubulin primary antibody and an Alexa Fluor 488 secondary antibody. Images were collected on a Zeiss Elyra Super-resolution microscope by Chris Rieken

Preparation: formaldehyde fixed tissue; glutaraldehyde fixed tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: structured illumination microscopy (SIM)
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: primary antibody plus labeled secondary antibody; Alexa Fluor 488
Processing history: maximum projection

• Cell Image Library Group ID: 9555

• Drosophila melanogaster

• Epithelial cell
• Microtubule

• Microtubule cytoskeleton organization
• Schneider S2

• data
• image

• No linguistic content; Not applicable

Samplenumber: 36147

Doi: https://doi.org/10.6075/J02N50XM

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL36147

Other resource

• MBL Neurobiology Course 2011 (Neurobiology): https://www.mbl.edu/education/advanced-research-training-courses/course-offerings

Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License

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2022-08-12