{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1313640000},"Data_type":{"Still_image":false,"Z_stack":true,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"36147.tif","Size":2200000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"36147.jpg","Size":156247,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"36147.zip","Size":2167619,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"OTHER":["MBL Neurobiology Course 2011"],"URLs":[{"Label":"MBL Neurobiology Course 2011","Href":"http:\/\/www.mbl.edu\/education\/courses\/summer\/course_neurobio.html"}],"Contributors":["Victor Cazares","Luke Chao","James A Galbraith","Catherine G Galbraith"]},"BIOLOGICALPROCESS":{"onto_name":"microtubule cytoskeleton organization","onto_id":"GO:0000226"},"PROCESSINGHISTORY":{"free_text":"maximum projection"},"CELLLINE":{"free_text":"Schneider S2"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"The first image in this multi-image tiff file is a structured illumination image (SIM) of microtubules in a Drosophila S2 cell.  The second image is the corresponding diffraction limited image obtained with conventional fluorescence microscopy.  Note the two-fold increase in spatial resolution in the SIM image compared to the diffraction limited image.  The z-series are available as CIL 36797.  Images were collected as part of the Marine Biological Laboratory Neurobiology Course, Summer 2011."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":{"onto_name":"microtubule","onto_id":"GO:0005874"},"RELATIONTOINTACTCELL":{"onto_name":"dispersed cells in vitro","onto_id":"FBbi:00000611"},"SOURCEOFCONTRAST":{"onto_name":"distribution of epitope","onto_id":"FBbi:00000592"},"GROUP_ID":"9555","TECHNICALDETAILS":{"free_text":"Cells were plated on Con-A coated coverslips, chemically fixed, and labeled with an anti-tubulin primary antibody and an Alexa Fluor 488 secondary antibody.  Images were collected on a Zeiss Elyra Super-resolution microscope by Chris Rieken"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"structured illumination microscopy (SIM)","onto_id":"FBbi:00000332"},"DIMENSION":[{"Space":{"Image_size":1054,"Pixel_size":{"unit":"microns","value":0.0397},"axis":"X"}},{"Space":{"Image_size":1028,"Pixel_size":{"unit":"microns","value":0.0397},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Drosophila melanogaster","onto_id":"NCBITaxon:7227"},"PREPARATION":[{"onto_name":"formaldehyde fixed tissue","onto_id":"FBbi:00000010"},{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"}],"VISUALIZATIONMETHODS":[{"onto_name":"primary antibody plus labeled secondary antibody","onto_id":"FBbi:00000156"},{"onto_name":"Alexa Fluor 488","onto_id":"FBbi:00000440"}],"CELLTYPE":{"onto_name":"epithelial cell","onto_id":"CL:0000066"}}},"Citation":{"Title":"Victor Cazares, Luke Chao, James A Galbraith, Catherine G Galbraith (2011) CIL:36147, Drosophila melanogaster, epithelial cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil36147","DOI":"doi:10.7295\/W9CIL36147"}}}