CIL:40702, Cricetulus griseus, epithelial cell
CIL source metadata (JSON)
File Size |
|
File Format |
|
- Collection
- Cite This Work
-
Ris, Hans (2021). CIL:40702, Cricetulus griseus, epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0C24VSQ
- Description
-
High voltage (1MeV) transmission electron microscopy image of an isolated metaphase chromatid pair from a Chinese hamster ovary cell, showing the fiber-like structure and two large pieces of attached membrane. The image was taken with a specimen tilt of 48 degrees. Grouped with it is an image with a tilt of 55 degrees, providing a pair that affords an oblique stereo view of the chromosome.
- Creation Date
- 1978-01-14
- Date Issued
- 2021
- Researcher
- Methods
-
Cells were treated with colchicine for 24 hr, and metaphase chromosomes isolated using the non-aqueous hexylene glycol protocol developed by Stubblefield and Wray, placed on a grid, critical point dried, and stained with uranyl acetate.
See also:
H. Ris 1981 Steroscopic electron microscopy of chromosomes. Meth Cell Biol 22:77-96
H. Ris 1978 Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:220-246. - Technical Details
-
Preparation: glutaraldehyde fixed tissue; critical_point dried specimen
Relation to intact cell: isolated subcellular component
Item type: film
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt
Data qualification: Raw;spatialmeasurements;intensitiesquantitation - Series
- Scientific Name
- Anatomy
- Topics
Formats
View formats within this collection
- Language
- No linguistic content; Not applicable
- Identifier
-
Samplenumber: 40702
- Related Resource
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL40702
Source data
- License
- Copyright
-
Creative Commons Public Domain Dedication
Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Constraint(s) on Use: This work may be used without prior permission.
- Digital Object Made Available By
-
Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2023-08-17