{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1339732800},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"40702.tif","Size":65400000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"40702.jpg","Size":5933468,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"40702.zip","Size":65336438,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"DATE":["01\/14\/1978"],"Contributors":["Hans Ris"]},"BIOLOGICALPROCESS":[{"onto_name":"chromosome organization","onto_id":"GO:0051276"},{"onto_name":"mitosis","onto_id":"GO:0007067"},{"onto_name":"mitotic metaphase","onto_id":"GO:0000089"}],"CELLLINE":{"free_text":"CHO"},"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"High voltage (1MeV) transmission electron microscopy image of an isolated metaphase chromatid pair from a Chinese hamster ovary cell, showing the fiber-like structure and two large pieces of attached membrane. The image was taken with a specimen tilt of 48 degrees. Grouped with it is an image with a tilt of 55 degrees, providing a pair that affords an oblique stereo view of the chromosome."},"ITEMTYPE":{"onto_name":"film","onto_id":"FBbi:00000303"},"CELLULARCOMPONENT":{"onto_name":"nuclear chromosome","onto_id":"GO:0000228"},"RELATIONTOINTACTCELL":{"onto_name":"isolated subcellular component","onto_id":"FBbi:00000579"},"SOURCEOFCONTRAST":{"onto_name":"differences in adsorption or binding of stain","onto_id":"FBbi:00000598"},"GROUP_ID":"11979","TECHNICALDETAILS":{"free_text":"Cells were treated with colchicine for 24 hr, and metaphase chromosomes isolated using the non-aqueous hexylene glycol  protocol developed by Stubblefield and Wray, placed on a grid, critical point dried, and stained with uranyl acetate.\nSee also:\nH. Ris 1981 Steroscopic electron microscopy of chromosomes. Meth Cell Biol 22:77-96\nH. Ris 1978 Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:220-246."},"DATAQUALIFICATION":{"free_text":"RAW;spatialmeasurements;intensitiesquantitation"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_id":"FBbi:00000622"},"DIMENSION":[{"Space":{"Image_size":4700,"Pixel_size":{"unit":"nanometers","value":1},"axis":"X"}},{"Space":{"Image_size":6950,"Pixel_size":{"unit":"nanometers","value":1},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Cricetulus griseus","onto_id":"NCBITaxon:10029"},"PREPARATION":[{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},{"onto_name":"critical_point dried specimen","onto_id":"FBbi:00000581"}],"VISUALIZATIONMETHODS":{"onto_name":"uranyl salt","onto_id":"FBbi:00000569"},"CELLTYPE":{"onto_name":"epithelial cell","onto_id":"CL:0000066"}}},"Citation":{"Title":"Hans Ris (2012) CIL:40702, Cricetulus griseus, epithelial cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil40702","DOI":"doi:10.7295\/W9CIL40702"}}}