CIL:13090, Xenopus [NCBITaxon:262014]
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- Cite This Work
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Maddox, Paul S.; Straight, Aaron; Coughlin, Peg; Mitchison, Timothy J.; Salmon, Edward D. (2021). CIL:13090, Xenopus [NCBITaxon:262014]. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0DZ0715
- Description
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Dual-channel spinning-disk confocal time-lapse movie showing kinetochores (Alexa488 labeled CENP-A antibody) in anaphase (red) relative to poleward microtubule flux. Fluorescent speckle microscopy (FSM) of microtubules and kinetochore imaging during anaphase was performed as described in Maddox et al. (2002, 2003), with a 100X/1.4 NA Plan Apochromat objective (Nikon) with 2X2 binning in the cooled CCD camera (model ER; Hamamatsu Corporation). MetaMorph® software (Universal Imaging Corp.) was used to control shutters, wavelength selection, image acquisition, and storage. Images were acquired at 5-s intervals. Sequential images of different fluorophores were acquired at 3-15s intervals, depending on the experiment. Paired images from different color channels were within 0.5 s of each other. Movie is video 3 from J Cell Biol 2003 162:377-382.
- Date Issued
- 2021
- Researchers
- Technical Details
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Preparation: unfixed tissue
Relation to intact cell: cell extract
Item type: recorded image
Imaging mode: spinning disk confocal microscopy; fluorescent speckle microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: X-Rhodamine; labeled primary antibody; Alexa Fluor 488
Processing history: color combine
Data qualification: Raw;intensitiesquantitation - Series
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- No linguistic content; Not applicable
- Identifier
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Samplenumber: 13090
- Related Resources
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL13090
- J Cell Biol 2003 162:377-382 https://www.ncbi.nlm.nih.gov/pubmed/?term=12900391
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- License
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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License
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- UC Regents
- Copyright
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Under copyright (US)
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12