{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1297314000},"Data_type":{"Still_image":false,"Z_stack":false,"Video":true,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"13090.zip","Size":16683136,"Mime_type":"application\/zip"},{"File_type":"Jpeg","File_path":"13090.jpg","Size":45965,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Mp4","File_path":"13090_web.mp4","Size":647982,"Mime_type":"video\/mp4"}],"CORE":{"ATTRIBUTION":{"PUBLISHED":["J Cell Biol 2003 162:377-382"],"Contributors":["Paul Maddox","Aaron Straight","Peg Coughlin","Timothy J. Mitchison","Edward D. Salmon"],"PUBMED":["12900391"]},"BIOLOGICALPROCESS":[{"onto_name":"mitotic anaphase","onto_id":"GO:0000090"},{"free_text":"poleward microtubule flux"}],"PROCESSINGHISTORY":{"free_text":"color combine"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Dual-channel spinning-disk confocal time-lapse movie showing kinetochores (Alexa488 labeled CENP-A antibody) in anaphase (red) relative to poleward microtubule flux. Fluorescent speckle microscopy (FSM) of microtubules and kinetochore imaging during anaphase was performed as described in Maddox et al. (2002, 2003), with a 100X\/1.4 NA Plan Apochromat objective (Nikon) with 2X2 binning in the cooled CCD camera (model ER; Hamamatsu Corporation). MetaMorph® software (Universal Imaging Corp.) was used to control shutters, wavelength selection, image acquisition, and storage.  Images were acquired at 5-s intervals. Sequential images of different fluorophores were acquired at 3-15s intervals, depending on the experiment. Paired images from different color channels were within 0.5 s of each other.  Movie is video 3 from J Cell Biol 2003 162:377-382."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"spindle microtubule","onto_id":"GO:0005876"},{"onto_name":"kinetochore","onto_id":"GO:0000776"}],"RELATIONTOINTACTCELL":{"free_text":"cell extract"},"SOURCEOFCONTRAST":{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},"GROUP_ID":"3925","DATAQUALIFICATION":{"free_text":"RAW;intensitiesquantitation"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":[{"onto_name":"spinning disk confocal microscopy","onto_id":"FBbi:00000253"},{"free_text":"fluorescent speckle microscopy"}],"DIMENSION":[{"Space":{"Image_size":431,"Pixel_size":{"unit":"microns","value":0.019},"axis":"X"}},{"Space":{"Image_size":364,"Pixel_size":{"unit":"microns","value":0.019},"axis":"Y"}},{"Wavelength":{"unit":"nanometers","value":"Alexa488, XRhodamine"}},{"Time":{"unit":"seconds","value":5}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Xenopus [NCBITaxon:262014]","onto_id":"NCBITaxon:262014"},"PREPARATION":{"onto_name":"unfixed tissue","onto_id":"FBbi:00000014"},"VISUALIZATIONMETHODS":[{"onto_name":"X-Rhodamine","onto_id":"FBbi:00000584"},{"onto_name":"labeled primary antibody","onto_id":"FBbi:00000130"},{"onto_name":"Alexa Fluor 488","onto_id":"FBbi:00000440"}]}},"Citation":{"Title":"Paul Maddox, Aaron Straight, Peg Coughlin, Timothy J. Mitchison, Edward D. Salmon (2011) CIL:13090, Xenopus [NCBITaxon:262014]. CIL. Dataset","ARK":"ark:\/b7295\/w9cil13090","DOI":"doi:10.7295\/W9CIL13090"}}}