Ris, Hans (2021). CIL:41609, Cricetulus griseus, epithelial cell, ovary. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0G73D26

High Voltage Transmission EM of whole mitotic metaphase chromosomes from Chinese hamster ovary cells. This image was recorded at a specimen tilt of 49 degrees and is grouped with one taken at 59 degrees, creating a stereo pair giving an oblique 3D view of the chromosomes.

• 1977-07-10

• 2021

• Ris, Hans

CHO cells arrested at metaphase with colcemid were treated with 67% D2O, in 0.07M phosphate buffer and squashed in 50% acetic acid. Preparations were transferred to EM grids and examined with the Wisconsin HVEM operated at 1MeV.
See also: H. Ris 1981 Stereoscopic electron microscopy of chromosomes. Meth Cell Biol 22:77-96 H. Ris 1978 Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:220-246.

Preparation: chemically fixed tissue
Relation to intact cell: squash preparation
Item type: film
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt
Data qualification: Raw;spatialmeasurements;intensitiesquantitation

• Cell Image Library Group ID: 12022

• Cricetulus griseus

• Epithelial cell
• Nuclear chromosome
• Ovary

• CHO
• Chromosome organization
• Mitosis
• Mitotic metaphase

• data
• image

• No linguistic content; Not applicable

Samplenumber: 41609

Doi: https://doi.org/10.6075/J0G73D26

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL41609

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2023-08-17