{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1340856000},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"41609.tif","Size":23900000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"41609.jpg","Size":4154658,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"41609.zip","Size":23832658,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"DATE":["07\/10\/1977"],"Contributors":["Hans Ris"]},"BIOLOGICALPROCESS":[{"onto_name":"chromosome organization","onto_id":"GO:0051276"},{"onto_name":"mitotic metaphase","onto_id":"GO:0000089"},{"onto_name":"mitosis","onto_id":"GO:0007067"}],"CELLLINE":{"free_text":"CHO"},"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"High Voltage Transmission EM of whole mitotic metaphase chromosomes from Chinese hamster ovary cells. This image was recorded at a specimen tilt of 49 degrees and is grouped with one taken at 59 degrees, creating a stereo pair giving an oblique 3D view of the chromosomes."},"ITEMTYPE":{"onto_name":"film","onto_id":"FBbi:00000303"},"CELLULARCOMPONENT":{"onto_name":"nuclear chromosome","onto_id":"GO:0000228"},"RELATIONTOINTACTCELL":{"free_text":"squash preparation"},"SOURCEOFCONTRAST":{"onto_name":"differences in adsorption or binding of stain","onto_id":"FBbi:00000598"},"GROUP_ID":"12022","TECHNICALDETAILS":{"free_text":"CHO cells arrested at metaphase with colcemid were treated with 67% D2O, in 0.07M phosphate buffer and squashed in 50% acetic acid.  Preparations were transferred to EM grids and examined with the Wisconsin HVEM operated at 1MeV.\nSee also: H. Ris 1981 Stereoscopic electron microscopy of chromosomes. Meth Cell Biol 22:77-96 H. Ris 1978 Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:220-246."},"DATAQUALIFICATION":{"free_text":"RAW;spatialmeasurements;intensitiesquantitation"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_id":"FBbi:00000622"},"DIMENSION":[{"Space":{"Image_size":2819,"Pixel_size":{"unit":"nanometers","value":2},"axis":"X"}},{"Space":{"Image_size":4226,"Pixel_size":{"unit":"nanometers","value":2},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Cricetulus griseus","onto_id":"NCBITaxon:10029"},"PREPARATION":{"onto_name":"chemically fixed tissue","onto_id":"FBbi:00000002"},"VISUALIZATIONMETHODS":{"onto_name":"uranyl salt","onto_id":"FBbi:00000569"},"CELLTYPE":[{"free_text":"ovary"},{"onto_name":"epithelial cell","onto_id":"CL:0000066"}]}},"Citation":{"Title":"Hans Ris (2012) CIL:41609, Cricetulus griseus, epithelial cell, ovary. CIL. Dataset","ARK":"ark:\/b7295\/w9cil41609","DOI":"doi:10.7295\/W9CIL41609"}}}