Oldenbourg, Rudolf; LaFountain, James R. (2021). CIL:9060, Nephrotoma suturalis, spermatocyte. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0RN36W3

The events of meiosis I and II in a living insect spermatocyte beginning with the breakdown of the nuclear envelope at diakinesis through to the completion of cytokinesis. Testes from the Crane fly Nephrotoma suturalis were observed with time-lapse polarization using a Nikon Microphot SA, equipped for liquid crystal polarized light microscopy (LC-PolScope, CRi, Woburn Massachusetts) 60x/1.4 PlanApo oil immersion objective, 1.4 NA oil imm. condenser, with 1.5x zoom lens. Image acquisition and processing Camera: Images captured every 2 min over 5 hr 18 min by a QImaging Retiga EXi CCD camera. Raw images were processed using a 5-frame algorithm (Shribak and Oldenbourg, 2003). Movie images represent computed retardance as gray values ranging from 0 nm retardance (black) to 2 nm retardance or higher (white).

• 2021

• LaFountain, James R.
• Oldenbourg, Rudolf

Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image; charge coupled device (CCD); Processed image based on 5 recorded images
Imaging mode: polarization microscopy
Parameter imaged: UV/visible/IR photons
Source of contrast: intrinsic birefringence
Visualization methods: Processed image based on 5 recorded images
Processing history: 5-frame algorithm

• Cell Image Library Group ID: 3782

• Nephrotoma suturalis

• Cytoskeleton
• Nuclear chromosome
• Spermatocyte
• Spindle

• Male meiosis chromosome segregation
• Spindle assembly involved in male meiosis

• data
• image
• video

• No linguistic content; Not applicable

Samplenumber: 9060

Doi: https://doi.org/10.6075/J0RN36W3

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL9060

Reference

• PubMed article: https://www.ncbi.nlm.nih.gov/pubmed/?term=15385630
• Shribak M, Oldenbourg R. Techniques for fast and sensitive measurements of two-dimensional birefringence distributions. Appl Opt. 2003 Jun 1;42(16):3009-17. https:/doi.org/10.1364/ao.42.003009

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2022-08-12