{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1289365200},"Data_type":{"Still_image":false,"Z_stack":false,"Video":true,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Jpeg","File_path":"9060.jpg","Size":60531,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"9060.zip","Size":13590652,"Mime_type":"application\/zip"},{"File_type":"Mp4","File_path":"9060_web.mp4","Size":1224481,"Mime_type":"video\/mp4"}],"CORE":{"ATTRIBUTION":{"Contributors":["Rudolf Oldenbourg","James R. LaFountain"],"PUBMED":["15385630","12790452"]},"BIOLOGICALPROCESS":[{"onto_name":"male meiosis chromosome segregation","onto_id":"GO:0007060"},{"onto_name":"spindle assembly involved in male meiosis","onto_id":"GO:0007053"}],"PROCESSINGHISTORY":{"free_text":"5-frame algorithm"},"PARAMETERIMAGED":{"onto_name":"UV\/visible\/IR photons","onto_id":"FBbi:00000378"},"IMAGEDESCRIPTION":{"free_text":"The events of meiosis I and II in a living insect spermatocyte beginning with the breakdown of the nuclear envelope at diakinesis through  to the completion of cytokinesis. Testes from the Crane fly Nephrotoma suturalis were observed with time-lapse polarization using a Nikon Microphot SA, equipped for liquid crystal polarized light microscopy (LC-PolScope, CRi, Woburn Massachusetts) 60x\/1.4 PlanApo oil immersion objective, 1.4 NA oil imm. condenser, with 1.5x zoom lens. Image acquisition and processing Camera: Images captured every 2 min over 5 hr 18 min by a QImaging Retiga EXi CCD camera. Raw images were processed using a 5-frame algorithm (Shribak and Oldenbourg, 2003). Movie images represent computed retardance as gray values ranging from 0 nm retardance (black) to 2 nm retardance or higher (white)."},"ITEMTYPE":[{"onto_name":"recorded image","onto_id":"FBbi:00000265"},{"onto_name":"charge coupled device (CCD)","onto_id":"FBbi:00000294"},{"free_text":"Processed image based on 5 recorded images"}],"CELLULARCOMPONENT":[{"onto_name":"cytoskeleton","onto_id":"GO:0005856"},{"onto_name":"nuclear chromosome","onto_id":"GO:0000228"},{"onto_name":"spindle","onto_id":"GO:0005819"}],"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":{"onto_name":"intrinsic birefringence","onto_id":"FBbi:00000594"},"GROUP_ID":"3782","TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"polarization microscopy","onto_id":"FBbi:00000248"},"DIMENSION":[{"Space":{"Image_size":500,"Pixel_size":{"unit":"nanometers","value":87},"axis":"X"}},{"Space":{"Image_size":500,"Pixel_size":{"unit":"nanometers","value":87},"axis":"Y"}},{"Time":{"unit":"seconds","value":120,"frame":"153"}}],"NCBIORGANISMALCLASSIFICATION":{"free_text":"Nephrotoma suturalis"},"PREPARATION":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"VISUALIZATIONMETHODS":{"free_text":"Processed image based on 5 recorded images"},"CELLTYPE":{"onto_name":"spermatocyte","onto_id":"CL:0000017"}}},"Citation":{"Title":"Rudolf Oldenbourg, James R. LaFountain (2010) CIL:9060, Nephrotoma suturalis, spermatocyte. CIL. Dataset","ARK":"ark:\/b7295\/w9cil9060","DOI":"doi:10.7295\/W9CIL9060"}}}