Ris, Hans; Olins, Ada; Fawcett, Don W. (2021). CIL:11828, Notophthalmus viridescens, Gallus gallus, erythrocyte. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0QJ7G38

In the upper panel, transmission electron microscopy reveals a tangled mass of ~30nm diameter nuclear chromatin fibers when the contents of the nucleus from erythrocytes of the newt Notophthalmus viridescens were spread on a water surface, fixed with formaldehyde, critical point dried, and metal shadowed. The lower panel shows chromatin from the nucleated erythrocyte of the chicken dispersed in low ionic strength, high pH buffer, centrifuged through a sucrose-formaldehyde cushion onto a carbon film and stained with uranyl acetate. When fully dispersed, the 'beads-on-a-string nucleosomal organization of chromatin is clearly evident.

• 2021

• Fawcett, Don W.
• Olins, Ada
• Ris, Hans

Figures 121 (upper panel, provided by Hans Ris) and 122 (lower, provided by Ada Olons) from Chapter 4 (Nucleus) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. A PDF copy of the chapter is available on the ASCB's BioEDUCATE website.

Preparation: critical_point dried specimen; formaldehyde fixed tissue
Relation to intact cell: isolated subcellular component
Item type: film
Imaging mode: transmission electron microscopy (TEM); detection of electrons
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain; differences in deposition of metal shadow
Visualization methods: uranyl salt
Processing history: Image extracted from .pdf file of 'The Cell'
Data qualification: Processed

• Cell Image Library Group ID: 11180

• Gallus gallus
• Notophthalmus viridescens

• Chromatin
• Erythrocyte
• Nucleosome

• Chromatin organization
• Nucleus organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 11828

Doi: https://doi.org/10.6075/J0QJ7G38

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL11828

Other resource

• Fawcett, Don W. "The Cell." 2nd ed., W.B. Saunders, 1981, ISBN 0721635849. https://www.ascb.org/career-development/teaching/don-fawcetts-the-cell/

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2022-08-12