{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1294203600},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"11828.tif","Size":3300000,"Mime_type":"image\/tif"},{"File_type":"Zip","File_path":"11828.zip","Size":3293948,"Mime_type":"application\/zip"},{"File_type":"Jpeg","File_path":"11828.jpg","Size":392192,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Label":"ASCB BioEDUCATE Program","Href":"http:\/\/www.ascb.org\/bioeducate\/FawcettTheCell.htm"}],"PUBLISHED":["ISBN 0721635849","from: Don W. Fawcett M.D  \u2018The Cell\u2019 2nd edition, W.B. Saunders  Chapter 4 (Nucleus)"],"Contributors":["Hans Ris","Ada Olins","Don W Fawcett"]},"BIOLOGICALPROCESS":[{"onto_name":"nucleus organization","onto_id":"GO:0006997"},{"onto_name":"chromatin organization","onto_id":"GO:0006325"}],"PROCESSINGHISTORY":{"free_text":"Image extracted from .pdf file of 'The Cell'"},"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"In the upper panel, transmission electron microscopy reveals a tangled mass of ~30nm diameter nuclear chromatin fibers when the contents of the nucleus from erythrocytes of the newt Notophthalmus viridescens were spread on a water surface, fixed with formaldehyde, critical point dried, and metal shadowed. The lower panel shows chromatin from the nucleated erythrocyte of the chicken dispersed in low ionic strength, high pH buffer, centrifuged through a sucrose-formaldehyde cushion onto a carbon film and stained with uranyl acetate. When fully dispersed, the 'beads-on-a-string nucleosomal organization of chromatin is clearly evident."},"ITEMTYPE":{"onto_name":"film","onto_id":"FBbi:00000303"},"CELLULARCOMPONENT":[{"onto_name":"chromatin","onto_id":"GO:0000785"},{"onto_name":"nucleosome","onto_id":"GO:0000786"}],"RELATIONTOINTACTCELL":{"onto_name":"isolated subcellular component","onto_id":"FBbi:00000579"},"SOURCEOFCONTRAST":[{"onto_name":"differences in adsorption or binding of stain","onto_id":"FBbi:00000598"},{"onto_name":"differences in deposition of metal shadow","onto_id":"FBbi:00000601"}],"GROUP_ID":"11180","TECHNICALDETAILS":{"free_text":"Figures 121 (upper panel, provided by Hans Ris) and 122 (lower, provided by Ada Olons) from Chapter 4 (Nucleus) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D.  A PDF copy of the chapter is available on the ASCB's BioEDUCATE website."},"DATAQUALIFICATION":{"free_text":"PROCESSED"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_nd"},"IMAGINGMODE":[{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},{"onto_name":"detection of electrons","onto_id":"FBbi:00000375"}],"DIMENSION":[{"Space":{"Image_size":865,"axis":"X"}},{"Space":{"Image_size":1268,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":[{"onto_name":"Notophthalmus viridescens","onto_id":"NCBITaxon:8316"},{"onto_name":"Gallus gallus","onto_id":"NCBITaxon:9031"}],"PREPARATION":[{"onto_name":"critical_point dried specimen","onto_id":"FBbi:00000581"},{"onto_name":"formaldehyde fixed tissue","onto_id":"FBbi:00000010"}],"VISUALIZATIONMETHODS":{"onto_name":"uranyl salt","onto_id":"FBbi:00000569"},"CELLTYPE":{"onto_name":"erythrocyte","onto_id":"CL:0000232"}}},"Citation":{"Title":"Hans Ris, Ada Olins, Don W Fawcett (2011) CIL:11828, Notophthalmus viridescens, Gallus gallus, erythrocyte. CIL. Dataset","ARK":"ark:\/b7295\/w9cil11828","DOI":"doi:10.7295\/W9CIL11828"}}}