Allen, Richard (2021). CIL:39181, Paramecium caudatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0J101TX

The cytoproct is the cell’s defecation site where spent digestive vacuoles fuse with the plasma membrane. In this cell the cytoproct is closed and forms a ridge along the posterior suture on the ventral side of the cell. The ridge contains distinctive fibers not found in other parts of the cell. It is covered on its sides by alveoli but has a single membrane over the crest of the ridge. The somatic basal bodies on both sides of the ridge give rise to microtubular bundles that extend into the endoplasm where they lie against the surface of approaching spent vacuoles (DV-IV). The spent vacuole has tubular extensions protruding from its membrane. TEM taken on 4/13/73 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 13,250X. Adapted with permission.

• 2021

• Allen, Richard

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; lead salt; osmium tetroxide
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation

• Cell Image Library Group ID: 10125

• Paramecium caudatum

• Cell by organism
• Cell cortex
• Ciliated Protist
• Cytoproct
• Eukaryotic cell
• Eukaryotic Protist
• Microtubule associated complex
• Vesicle membrane

• Cortical cytoskeleton organization
• Digestion
• Digestive system process

• data
• image

• No linguistic content; Not applicable

Samplenumber: 39181

Doi: https://doi.org/10.6075/J0J101TX

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL39181

Reference

• J Cell Sci. 1974 May;14(3):611-31. https://www.ncbi.nlm.nih.gov/pubmed/?term=4364579

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2022-08-12