{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1322974800},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"39181.zip","Size":46924350,"Mime_type":"application\/zip"},{"File_type":"Jpeg","File_path":"39181.jpg","Size":5638437,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"OME_tif","File_path":"39181.tif","Size":47000000,"Mime_type":"image\/tif"}],"CORE":{"ATTRIBUTION":{"PUBLISHED":["J Cell Sci. 1974 May;14(3):611-31."],"Contributors":["Richard Allen (University of Hawaii)"],"PUBMED":["4364579"]},"BIOLOGICALPROCESS":[{"onto_name":"digestion","onto_id":"GO:0007586"},{"onto_name":"digestive system process","onto_id":"GO:0022600"},{"onto_name":"cortical cytoskeleton organization","onto_id":"GO:0030865"}],"PROCESSINGHISTORY":[{"onto_name":"recorded image","onto_id":"FBbi:00000265"},{"onto_name":"film","onto_id":"FBbi:00000303"},{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"}],"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"The cytoproct is the cell\u2019s defecation site where spent digestive vacuoles fuse with the plasma membrane. In this cell the cytoproct is closed and forms a ridge along the posterior suture on the ventral side of the cell. The ridge contains distinctive fibers not found in other parts of the cell. It is covered on its sides by alveoli but has a single membrane over the crest of the ridge. The somatic basal bodies on both sides of the ridge give rise to microtubular bundles that extend into the endoplasm where they lie against the surface of approaching spent vacuoles (DV-IV). The spent vacuole has tubular extensions protruding from its membrane. TEM taken on 4\/13\/73 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 13,250X. Adapted with permission."},"ITEMTYPE":[{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},{"onto_name":"illumination by electrons","onto_id":"FBbi:00000273"}],"CELLULARCOMPONENT":[{"onto_name":"cytoproct","onto_id":"GO:0031911"},{"onto_name":"microtubule associated complex","onto_id":"GO:0005875"},{"onto_name":"cell cortex","onto_id":"GO:0005938"},{"onto_name":"vesicle membrane","onto_id":"GO:0012506"}],"RELATIONTOINTACTCELL":{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"},"SOURCEOFCONTRAST":{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},"GROUP_ID":"10125","TECHNICALDETAILS":{"free_text":"Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http:\/\/www5.pbrc.hawaii.edu\/allen\/)."},"DATAQUALIFICATION":{"free_text":"RAW;spatialmeasurements;intensitiesquantitation"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":[{"onto_name":"detection of electrons","onto_id":"FBbi:00000375"},{"onto_name":"film","onto_id":"FBbi:00000303"}],"DIMENSION":[{"Space":{"Image_size":4572,"Pixel_size":{"unit":"nanometers","value":1.1},"axis":"X"}},{"Space":{"Image_size":5131,"Pixel_size":{"unit":"nanometers","value":1.1},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Paramecium caudatum","onto_id":"NCBITaxon:5885"},"PREPARATION":[{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},{"onto_name":"osmium tetroxide fixed tissue","onto_id":"FBbi:00000012"},{"onto_name":"tissue in epoxy resin embedment","onto_id":"FBbi:00000018"},{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"}],"VISUALIZATIONMETHODS":[{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},{"onto_name":"uranyl salt","onto_id":"FBbi:00000569"},{"onto_name":"lead salt","onto_id":"FBbi:00000570"},{"onto_name":"osmium tetroxide","onto_id":"FBbi:00000571"}],"CELLTYPE":[{"onto_name":"cell by organism","onto_id":"CL:0000004"},{"onto_name":"eukaryotic cell","onto_id":"CL:0000255"},{"free_text":"Eukaryotic Protist"},{"free_text":"Ciliated Protist"}]}},"Citation":{"Title":"Richard Allen (University of Hawaii) (2011) CIL:39181, Paramecium caudatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset","ARK":"ark:\/b7295\/w9cil39181","DOI":"doi:10.7295\/W9CIL39181"}}}