Pasapera, Ana M.; Waterman, Clare M. (2021). CIL:7439, Mus musculus, fibroblast, primary cell line cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J01G0K86

Mouse Embryonic Fibroblasts (MEFs) grown on glass coverslips coated with 10 ug/ml Fibronectin. After 24 hrs cells were fixed in 3% paraformaldehyde and stained with mouse antibody to Paxillin, rabbit antibody to cortactin, followed by anti-mouse Alexa 561 goat antibody for Cortactin (cblue), anti-rabbit Alexa 647 for Paxillin (green) and Alexa 488 phalloidin (red) to stain for F-actin.

Images were collected on a Nikon Eclipse imaged with a cooled charge coupled device (CCD; Orca II; Hamamatsu Photonics) using a 60x 1.4 NA Plan Apo PH objective lens (Nikon). Individual exposure times were used for each channel: 488: 200ms, 568: 200 ms, 647: 50 ms.

• 2021

• Pasapera, Ana M.
• Waterman, Clare M.

Preparation: formaldehyde fixed tissue; detergent permeabilized
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: Alexa Fluor 488; Alexa Fluor 568; Alexa Fluor 647; primary antibody plus labeled secondary antibody
Processing history: unprocessed raw data

• Mus musculus

• Actin cytoskeleton
• Fibroblast
• Focal adhesion
• Primary cell line cell

• Actin binding
• Actin filament polymerization
• Cell adhesion
• Cell adhesion molecule binding

• data
• image

• No linguistic content; Not applicable

Samplenumber: 7439

Doi: https://doi.org/10.6075/J01G0K86

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL7439

Reference

• PubMed article: https://www.ncbi.nlm.nih.gov/pubmed/?term=20308429

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2022-08-12