{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1288152000},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Jpeg","File_path":"7439.jpg","Size":281820,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"OME_tif","File_path":"7439.tif","Size":4400000,"Mime_type":"image\/tif"},{"File_type":"Zip","File_path":"7439.zip","Size":4357874,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"OTHER":["NHLBI\/ NIH"],"Contributors":["Ana M. Pasapera","Clare M. Waterman"],"PUBMED":["20308429"]},"BIOLOGICALPROCESS":[{"onto_name":"cell adhesion","onto_id":"GO:0007155"},{"onto_name":"actin filament polymerization","onto_id":"GO:0030041"}],"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Mouse Embryonic Fibroblasts (MEFs) grown on glass coverslips coated with 10 ug\/ml  Fibronectin. After 24 hrs cells were fixed in 3% paraformaldehyde and stained with mouse antibody to Paxillin, rabbit antibody to cortactin, followed by anti-mouse Alexa 561 goat antibody for Cortactin (cblue), anti-rabbit Alexa 647 for Paxillin (green) and Alexa 488 phalloidin (red)  to stain for F-actin. \n\nImages were collected on a Nikon Eclipse imaged with a cooled charge coupled device (CCD; Orca II; Hamamatsu Photonics) using a 60x 1.4 NA Plan Apo PH objective lens (Nikon).  Individual exposure times were used for each channel: 488: 200ms, 568: 200 ms, 647: 50 ms."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"focal adhesion","onto_id":"GO:0005925"},{"onto_name":"actin cytoskeleton","onto_id":"GO:0015629"}],"RELATIONTOINTACTCELL":{"onto_name":"dispersed cells in vitro","onto_id":"FBbi:00000611"},"SOURCEOFCONTRAST":{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},"MOLECULARFUNCTION":[{"onto_name":"actin binding","onto_id":"GO:0003779"},{"onto_name":"cell adhesion molecule binding","onto_id":"GO:0050839"}],"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"fluorescence microscopy","onto_id":"FBbi:00000246"},"DIMENSION":[{"Space":{"Image_size":1392,"Pixel_size":{"unit":"nanometers","value":0.107},"axis":"X"}},{"Space":{"Image_size":1040,"Pixel_size":{"unit":"microns","value":0.107},"axis":"Y"}},{"Wavelength":{"unit":"nanometers","value":"488"}},{"Wavelength":{"unit":"nanometers","value":"568"}},{"Wavelength":{"unit":"nanometers","value":"647"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Mus musculus","onto_id":"NCBITaxon:10090"},"PREPARATION":[{"onto_name":"formaldehyde fixed tissue","onto_id":"FBbi:00000010"},{"onto_name":"detergent permeabilized","onto_id":"FBbi:00000262"}],"VISUALIZATIONMETHODS":[{"onto_name":"Alexa Fluor 488","onto_id":"FBbi:00000440"},{"onto_name":"Alexa Fluor 568","onto_id":"FBbi:00000442"},{"onto_name":"Alexa Fluor 647","onto_id":"FBbi:00000447"},{"onto_name":"primary antibody plus labeled secondary antibody","onto_id":"FBbi:00000156"}],"CELLTYPE":[{"onto_name":"fibroblast","onto_id":"CL:0000057"},{"onto_name":"primary cell line cell","onto_id":"CL:0000001"}]}},"Citation":{"Title":"Ana M. Pasapera, Clare M. Waterman (2010) CIL:7439, Mus musculus, fibroblast, primary cell line cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil7439","DOI":"doi:10.7295\/W9CIL7439"}}}