Mahjoub, Moe; Stearns, Tim (2021). CIL:10061, Mus musculus, tracheal epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J05X2845

Mouse tracheal epithelia cells (MTEC) were grown at air-liquid interface to induce differentiation, centriole amplification and ciliogenesis. Cells were fixed with ice-cold methanol for 10 min. Samples were stained with antibodies against acetylated tubulin to mark cilia (red), the centriolar protein Cep120 to mark basal bodies (green), and anti-ZO-1 to mark the cell-cell junctions (light blue). Secondary antibodies were Alexa 594 for acetylated tubulin, Alexa 488 for Cep120, and Cy5 for Zo-1. Nuclei were stained using DAPI (blue). Images were captured using Openlab 4.0.4 software controlling an Axiovert 200M microscope (Carl Zeiss, Inc.) with a 100X 1.4 NA objective.

• 2021

• Mahjoub, Moe
• Stearns, Tim

Preparation: methanol fixed tissue
Relation to intact cell: detergent permeabilized
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific nucleic acid sequence
Visualization methods: primary antibody plus labeled secondary antibody; 4',6-diamidino-2-phenylindole (DAPI); Alexa Fluor 488; Alexa Fluor 594; Cy5
Processing history: unprocessed raw data

• Cell Image Library Group ID: 8084

• Mus musculus

• Acetylated tubulin
• Cell-cell junction
• Microtubule basal body
• Nucleus
• Tracheal epithelial cell

• Lung ciliated cell differentiation

• data
• image

• No linguistic content; Not applicable

Samplenumber: 10061

Doi: https://doi.org/10.6075/J05X2845

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL10061

CC0 1.0 Universal Public Domain Dedication

Creative Commons Public Domain Dedication

Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.

Constraint(s) on Use: This work may be used without prior permission.

Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12