{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1293080400},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"10061.tif","Size":3600000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"10061.jpg","Size":194892,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"10061.zip","Size":3604226,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"Contributors":["Moe Mahjoub","Tim Stearns"]},"BIOLOGICALPROCESS":{"onto_name":"lung ciliated cell differentiation","onto_id":"GO:0061141"},"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Mouse tracheal epithelia cells (MTEC) were grown at air-liquid interface to induce differentiation, centriole amplification and ciliogenesis. Cells were fixed with ice-cold methanol for 10 min. Samples were stained with antibodies against acetylated tubulin to mark cilia (red), the centriolar protein Cep120 to mark basal bodies (green), and anti-ZO-1 to mark the cell-cell junctions (light blue). Secondary antibodies were Alexa 594 for acetylated tubulin, Alexa 488 for Cep120, and Cy5 for Zo-1.  Nuclei were stained using DAPI (blue). Images were captured using Openlab 4.0.4 software controlling an Axiovert 200M microscope (Carl Zeiss, Inc.) with a 100X 1.4 NA objective."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"cell-cell junction","onto_id":"GO:0005911"},{"onto_name":"microtubule basal body","onto_id":"GO:0005932"},{"onto_name":"nucleus","onto_id":"GO:0005634"},{"free_text":"acetylated tubulin"}],"RELATIONTOINTACTCELL":{"onto_name":"detergent permeabilized","onto_id":"FBbi:00000262"},"SOURCEOFCONTRAST":{"onto_name":"distribution of a specific nucleic acid sequence","onto_id":"FBbi:00000596"},"GROUP_ID":"8084","TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"fluorescence microscopy","onto_id":"FBbi:00000246"},"DIMENSION":[{"Space":{"Image_size":1171,"Pixel_size":{"unit":"microns","value":0.094},"axis":"X"}},{"Space":{"Image_size":1017,"Pixel_size":{"unit":"microns","value":0.094},"axis":"Y"}},{"Wavelength":{"note":"4","unit":"nanometers","value":"350, 488, 594, 633"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Mus musculus","onto_id":"NCBITaxon:10090"},"PREPARATION":{"onto_name":"methanol fixed tissue","onto_id":"FBbi:00000007"},"VISUALIZATIONMETHODS":[{"onto_name":"primary antibody plus labeled secondary antibody","onto_id":"FBbi:00000156"},{"onto_name":"4',6-diamidino-2-phenylindole (DAPI)","onto_id":"FBbi:00000056"},{"onto_name":"Alexa Fluor 488","onto_id":"FBbi:00000440"},{"onto_name":"Alexa Fluor 594","onto_id":"FBbi:00000444"},{"onto_name":"Cy5","onto_id":"FBbi:00000450"}],"CELLTYPE":{"onto_name":"tracheal epithelial cell","onto_id":"CL:0000307"}}},"Citation":{"Title":"Moe Mahjoub, Tim Stearns (2010) CIL:10061, Mus musculus, tracheal epithelial cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil10061","DOI":"doi:10.7295\/W9CIL10061"}}}