Lam, Wai Yan; Rice, Marian (2021). CIL:27225, Fundulus heteroclitus, epidermal cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0JQ0ZZS

Cell junctions formed between adjacent epidermal cells in Fundulus heteroclitus scales. Desmosomes line the membranes of two epidermal cells. A vast network of intermediate filaments and microfilaments are present throughout the cytoplasm and connect to the desmosomes plates. Microtubules are also visible in the cells.

• 2021

• Lam, Wai Yan
• Rice, Marian

Fundulus heteroclitus scales were chemically fixed with 2.5% glutaraldehyde, 2% formaldehyde in 0.1M cacodylate buffer (pH 7.3), then post-fixed in 4% osmium tetroxide and stained en bloc in 1% uranyl acetate. The scales were then dehydrated in a graded series of ethanol and infiltrated with Spurr's resin. Thin sections of 70 nm were trimmed using a diamond knife and post-stained in uranyl acetate and lead citrate. This micrograph was imaged using a Phillips CM 100 transmission electron microscope at an accelerating voltage of 80 kV.

Preparation: glutaraldehyde fixed tissue; formaldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; dehydrated in ethanol
Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt; lead salt
Processing history: unprocessed raw data

• Cell Image Library Group ID: 9597

• Fundulus heteroclitus

• Cell-cell junction
• Desmosome
• Epidermal cell
• Intermediate filament
• Microfilament
• Microtubule

• Cell-cell junction organization
• Cytoskeleton organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 27225

Doi: https://doi.org/10.6075/J0JQ0ZZS

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL27225

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2022-08-12