{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1303531200},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"27225.zip","Size":6200730,"Mime_type":"application\/zip"},{"File_type":"OME_tif","File_path":"27225.tif","Size":6200000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"27225.jpg","Size":1120024,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"Contributors":["Wai Yan Lam","Marian Rice"]},"BIOLOGICALPROCESS":[{"onto_name":"cytoskeleton organization","onto_id":"GO:0007010"},{"onto_name":"cell-cell junction organization","onto_id":"GO:0045216"}],"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"Cell junctions formed between adjacent epidermal cells in Fundulus heteroclitus scales. Desmosomes line the membranes of two epidermal cells. A vast network of intermediate filaments and microfilaments are present throughout the cytoplasm and connect to the desmosomes plates. Microtubules are also visible in the cells."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"cell-cell junction","onto_id":"GO:0005911"},{"onto_name":"desmosome","onto_id":"GO:0030057"},{"onto_name":"intermediate filament","onto_id":"GO:0005882"},{"free_text":"microfilament"},{"onto_name":"microtubule","onto_id":"GO:0005874"}],"RELATIONTOINTACTCELL":{"onto_name":"sectioned tissue","onto_id":"FBbi:00000026"},"SOURCEOFCONTRAST":{"onto_name":"differences in adsorption or binding of stain","onto_id":"FBbi:00000598"},"GROUP_ID":"9597","TECHNICALDETAILS":{"free_text":"Fundulus heteroclitus scales were chemically fixed with 2.5% glutaraldehyde, 2% formaldehyde in 0.1M cacodylate buffer (pH 7.3), then post-fixed in 4% osmium tetroxide and stained en bloc in 1% uranyl acetate. The scales were then dehydrated in a graded series of ethanol and infiltrated with Spurr\u2019s resin. Thin sections of 70 nm were trimmed using a diamond knife and post-stained in uranyl acetate and lead citrate. This micrograph was imaged using a Phillips CM 100 transmission electron microscope at an accelerating voltage of 80 kV."},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},"DIMENSION":[{"Space":{"Image_size":1706,"Pixel_size":{"unit":"nanometers","value":1.92},"axis":"X"}},{"Space":{"Image_size":1808,"Pixel_size":{"unit":"nanometers","value":1.92},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Fundulus heteroclitus","onto_id":"NCBITaxon:8078"},"PREPARATION":[{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},{"onto_name":"formaldehyde fixed tissue","onto_id":"FBbi:00000010"},{"onto_name":"osmium tetroxide fixed tissue","onto_id":"FBbi:00000012"},{"free_text":"dehydrated in ethanol"},{"onto_name":"tissue in epoxy resin embedment","onto_id":"FBbi:00000018"}],"VISUALIZATIONMETHODS":[{"onto_name":"uranyl salt","onto_id":"FBbi:00000569"},{"onto_name":"lead salt","onto_id":"FBbi:00000570"}],"CELLTYPE":{"onto_name":"epidermal cell","onto_id":"CL:0000362"}}},"Citation":{"Title":"Wai Yan Lam, Marian Rice (2011) CIL:27225, Fundulus heteroclitus, epidermal cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil27225","DOI":"doi:10.7295\/W9CIL27225"}}}