Chen, Yi-Chun Maria (2021). CIL:37338, Mus musculus, fibroblast. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0SQ8ZQV

Promyelocytic leukemia nuclear bodies (PML NBs) are dynamic nuclear organelles. They range from 0.1um to 1um in size and exist in most of mammalian cell types. The functions of PML NBs are indicated by their association with a variety of component proteins that are involved in different cellular process. Here shows PML NBs labeled by monoclonal antibody (PML36) in 3T3 cells. .

• 2021

• Chen, Yi-Chun Maria

Cells were rinsed in PBS once and fixed for 15 min in 2% formaldehyde in PBS, pH 7.4. After being washed in PBS for 10 min x3, cells were permeablized 5 min in PBS with 0.2% Triton X-100 and 1% normal goat serum. After being washed in PBS plus 1% goat serum for 10 min x3, the following primary antibody (PML36) were added for 1 h at RT. Cells were rinsed in PBS with 1% normal goat serum for 10 min x3, and secondary FITC anti-mouse antibodies were added for 1 h at RT. Cells were rinsed in PBS for 10 min x3 and were observed on a modified Zeiss Axiovert 200M microscope with a plan-neofluar ×40 objective.
The microscope was fitted with dual excitation and emission filter wheels and a Photometrics Coolsnap-HQ camera.

Preparation: detergent permeabilized; formaldehyde fixed tissue
Relation to intact cell: dispersed cells in vitro
Item type: fluorescence microscopy
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: compartmentalization of stain or label; distribution of epitope
Visualization methods: Fluorescein (FITC); primary antibody plus labeled secondary antibody
Processing history: maximum projection
Data qualification: Processed

• Cell Image Library Group ID: 9278

• Mus musculus

• Fibroblast
• Nucleus
• PML body

• Apoptotic process
• NIH/3T3
• Response to virus
• Tumor necrosis factor-mediated signaling pathway

• data
• image

• No linguistic content; Not applicable

Samplenumber: 37338

Doi: https://doi.org/10.6075/J0SQ8ZQV

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL37338

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2022-08-12