{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1314849600},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Jpeg","File_path":"37338.jpg","Size":6512,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"OME_tif","File_path":"37338.tif","Size":300000,"Mime_type":"image\/tif"},{"File_type":"Zip","File_path":"37338.zip","Size":233274,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"Contributors":["Yi-Chun Maria Chen"]},"BIOLOGICALPROCESS":[{"onto_id":"GO:0090046"},{"onto_name":"response to virus","onto_id":"GO:0009615"},{"onto_name":"apoptotic process","onto_id":"GO:0006915"},{"onto_name":"tumor necrosis factor-mediated signaling pathway","onto_id":"GO:0033209"}],"PROCESSINGHISTORY":{"free_text":"maximum projection"},"CELLLINE":{"onto_name":"NIH\/3T3","onto_id":"MCC:0000362"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Promyelocytic leukemia nuclear bodies (PML NBs) are dynamic nuclear organelles. They range from 0.1um to 1um in size and exist in most of mammalian cell types. The functions of PML NBs  are indicated by their association with a variety of component proteins that are involved in different cellular process. Here shows PML NBs labeled by monoclonal antibody (PML36) in 3T3 cells.   ."},"ITEMTYPE":{"onto_name":"fluorescence microscopy","onto_id":"FBbi:00000246"},"CELLULARCOMPONENT":[{"onto_name":"nucleus","onto_id":"GO:0005634"},{"onto_name":"PML body","onto_id":"GO:0016605"}],"RELATIONTOINTACTCELL":{"onto_name":"dispersed cells in vitro","onto_id":"FBbi:00000611"},"SOURCEOFCONTRAST":[{"onto_name":"compartmentalization of stain or label","onto_id":"FBbi:00000604"},{"onto_name":"distribution of epitope","onto_id":"FBbi:00000592"}],"GROUP_ID":"9278","TECHNICALDETAILS":{"free_text":"Cells were rinsed in PBS once and fixed for 15 min in 2% formaldehyde in PBS, pH 7.4. After being washed in PBS for 10 min x3, cells were permeablized 5 min in PBS with 0.2% Triton X-100 and 1% normal goat serum. After being washed in PBS plus 1% goat serum for 10 min x3, the following primary antibody (PML36) were added for 1 h at RT. Cells were rinsed in PBS with 1% normal goat serum for 10 min x3, and secondary FITC anti-mouse antibodies were added for 1 h at RT. Cells were rinsed in PBS for 10 min x3 and were observed on a modified Zeiss Axiovert 200M microscope with a plan-neofluar ×40 objective.\nThe microscope was fitted with dual excitation and emission filter wheels and a Photometrics Coolsnap-HQ camera."},"DATAQUALIFICATION":{"free_text":"PROCESSED"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"fluorescence microscopy","onto_id":"FBbi:00000246"},"DIMENSION":[{"Space":{"Image_size":292,"Pixel_size":{"unit":"microns","value":0.16},"axis":"X"}},{"Space":{"Image_size":240,"Pixel_size":{"unit":"microns","value":0.16},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Mus musculus","onto_id":"NCBITaxon:10090"},"PREPARATION":[{"onto_name":"detergent permeabilized","onto_id":"FBbi:00000262"},{"onto_name":"formaldehyde fixed tissue","onto_id":"FBbi:00000010"}],"VISUALIZATIONMETHODS":[{"onto_name":"Fluorescein (FITC)","onto_id":"FBbi:00000451"},{"onto_name":"primary antibody plus labeled secondary antibody","onto_id":"FBbi:00000156"}],"CELLTYPE":{"onto_name":"fibroblast","onto_id":"CL:0000057"},"PATHOLOGICALPROCESS":{"onto_id":"IDO:IDO:0000096"}}},"Citation":{"Title":"Yi-Chun Maria Chen (2011) CIL:37338, Mus musculus, fibroblast. CIL. Dataset","ARK":"ark:\/b7295\/w9cil37338","DOI":"doi:10.7295\/W9CIL37338"},"Microbial_type":["virus"]}}