Mettlen, Marcel; Schmid, Sandra L. (2021). CIL:10136, Homo sapiens, epithelial cell, human retinal pigmented epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0DN4454

Visualization of clathrin coated pit dynamics. Total internal reflection fluorescencec microscopy (TIRF-FM) was performed on live human retinal pigment epithelial (RPE) cells stably expressing 'clathrin light chain a' tagged with EGFP (EGFP-LCa) using a 100x 1.45 NA objective (Nikon) mounted on a Nikon TE2000U inverted microscope (Nikon) coupled to a 14-bit mode operated Hamamatsu Orca II-ERG camera. This single channel TIR-FM movie, showing clathrin coated pit dynamics at the ventral plasma membrane, was acquired at a frame rate of 1s with a 70 msec exposure time during 10 min. Pixel size: 67 nm

• 2021

• Mettlen, Marcel
• Schmid, Sandra L.

Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: TIRF
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EGFP
Processing history: unprocessed raw data

• Homo sapiens

• Clathrin coat of coated pit
• Clathrin light chain a
• Epithelial cell
• Human retinal pigmented epithelial cell

• Clathrin coated pit dynamics

• data
• image

• No linguistic content; Not applicable

Samplenumber: 10136

Doi: https://doi.org/10.6075/J0DN4454

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL10136

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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12