{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1292648400},"Data_type":{"Still_image":false,"Z_stack":true,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Jpeg","File_path":"10136.jpg","Size":217150,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"OME_tif","File_path":"10136.tif","Size":730000000,"Mime_type":"image\/tif"},{"File_type":"Zip","File_path":"10136.zip","Size":729055413,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"OTHER":["The Scripps Research Institute"],"Contributors":["Marcel Mettlen","Sandra L. Schmid"]},"BIOLOGICALPROCESS":{"free_text":"clathrin coated pit dynamics"},"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Visualization of clathrin coated pit dynamics. Total internal reflection fluorescencec microscopy (TIRF-FM) was performed on live human retinal pigment epithelial (RPE) cells stably expressing 'clathrin light chain a' tagged with EGFP (EGFP-LCa) using a 100x 1.45 NA objective (Nikon) mounted on a Nikon TE2000U inverted microscope (Nikon) coupled to a 14-bit mode operated Hamamatsu Orca II-ERG camera. This single channel TIR-FM movie, showing clathrin coated pit dynamics at the ventral plasma membrane, was acquired at a frame rate of 1s with a 70 msec exposure time during 10 min. Pixel size: 67 nm"},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"clathrin coat of coated pit","onto_id":"GO:0030132"},{"free_text":"clathrin light chain a"}],"RELATIONTOINTACTCELL":{"onto_name":"dispersed cells in vitro","onto_id":"FBbi:00000611"},"SOURCEOFCONTRAST":{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"free_text":"TIRF"},"DIMENSION":[{"Space":{"Image_size":613,"Pixel_size":{"unit":"microns","value":67},"axis":"X"}},{"Space":{"Image_size":991,"Pixel_size":{"unit":"microns","value":67},"axis":"Y"}},{}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Homo sapiens","onto_id":"NCBITaxon:9606"},"PREPARATION":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"VISUALIZATIONMETHODS":{"onto_name":"EGFP","onto_id":"FBbi:00000082"},"CELLTYPE":[{"onto_name":"epithelial cell","onto_id":"CL:0000066"},{"free_text":"human retinal pigmented epithelial cell"}]}},"Citation":{"Title":"Marcel Mettlen, Sandra L. Schmid (2010) CIL:10136, Homo sapiens, epithelial cell, human retinal pigmented epithelial cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil10136","DOI":"doi:10.7295\/W9CIL10136"}}}