Shapiro, Lucy; McAdams, Harley (2021). CIL:39994, Caulobacter crescentus CB15. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0TT4R2P

A single slice from the slice-by-slice reconstructed volume of a Caulobacter Crescentus specimen. This particular slice is taken from Z slice # 142. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database.

• 2021

• McAdams, Harley
• Shapiro, Lucy

Caulobacter cultures (strain 3724) were cryofixed using a cocktail of 2% Osmium tetroxide, 0.5% uranyl acetate in anhydrous acetone following this time and temperature schedule: -90 Celsius for 72 hours, raised 2 Celsius per hour for 15 hours, held at -60 Celsius for 12 hours, raised 5 Celsius per hour for 12 hours, held at -20 Celsius. Film images were acquired using a JEOL 4000, at a magnification of 30,000X, 400.0 Kv, with the objective aperture set at 2.

Preparation: osmium tetroxide fixed tissue; cryofixed tissue
Item type: transmission electron microscopy (TEM)
Imaging mode: illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: uranyl salt
Processing history: recorded image
Data qualification: Processed

• Cell Image Library Group ID: 11209

• Caulobacter crescentus CB15

• data
• image

• No linguistic content; Not applicable

Samplenumber: 39994

Doi: https://doi.org/10.6075/J0TT4R2P

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL39994

Previous version

• Item in Cell Centered Database, UC San Diego Library Digital Collections: https://doi.org/10.6075/J0HM5883

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2022-08-12