{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1332734400},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"39994.tif","Size":800000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"39994.jpg","Size":65261,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Label":"CCDB 3566","Href":"http:\/\/ccdb.ucsd.edu\/sand\/main?event=displayAll&mpid=3566"}],"Contributors":["Lucy Shapiro"," Harley McAdams"]},"PROCESSINGHISTORY":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"A single slice from the slice-by-slice reconstructed volume of a Caulobacter Crescentus specimen.  This particular slice is taken from Z slice # 142. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database."},"ITEMTYPE":{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},"SOURCEOFCONTRAST":{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},"GROUP_ID":"11209","TECHNICALDETAILS":{"free_text":"Caulobacter cultures (strain 3724) were cryofixed using a cocktail of 2% Osmium tetroxide, 0.5% uranyl acetate in anhydrous acetone following this time and temperature schedule: -90 Celsius for 72 hours, raised 2 Celsius per hour for 15 hours, held at -60 Celsius for 12 hours, raised 5 Celsius per hour for 12 hours, held at -20 Celsius.  Film images were acquired using a JEOL 4000, at a magnification of 30,000X, 400.0 Kv, with the objective aperture set at 2."},"DATAQUALIFICATION":{"free_text":"PROCESSED"},"TERMSANDCONDITIONS":{"free_text":"attribution_cc_by"},"IMAGINGMODE":[{"onto_name":"illumination by electrons","onto_id":"FBbi:00000273"},{"onto_name":"detection of electrons","onto_id":"FBbi:00000375"}],"DIMENSION":[{"Space":{"Image_size":512,"axis":"X"}},{"Space":{"Image_size":512,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Caulobacter crescentus CB15","onto_id":"NCBITaxon:190650"},"PREPARATION":[{"onto_name":"osmium tetroxide fixed tissue","onto_id":"FBbi:00000012"},{"onto_name":"cryofixed tissue","onto_id":"FBbi:00000013"}],"VISUALIZATIONMETHODS":{"onto_name":"uranyl salt","onto_id":"FBbi:00000569"}}},"Citation":{"Title":"Lucy Shapiro,  Harley McAdams (2012) CIL:39994, Caulobacter crescentus CB15. CIL. Dataset","ARK":"ark:\/b7295\/w9cil39994","DOI":"doi:10.7295\/W9CIL39994"}}}