Ball, David; Peccoud, Jean (2021). CIL:39546, Saccharomyces cerevisiae. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0F76BWZ

This is a time lapse image series of Saccharomyces cerevisiae expressing GFP tagged Bub2, a GTPase activator involves in mitotic cell cycle spindle orientation checkpoint and regulation of exit from mitosis. These phase and GFP z-series images are part of an image group containing CIL 39546-39550. Note that there are additional groups of images of other cell cycle regulator proteins by the same authors available in the Library.

• 2021

• Ball, David
• Peccoud, Jean

Images were acquired using a 60x 1.42 NA objective lens on a Delta Vision system equipped with a CoolSNAP_HQ2 / HQ2-ICX285. Please read the microscopy section of the referenced paper for details before image analysis.

Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy; phase contrast microscopy
Parameter imaged: fluorescence emission; elastic scattering of photons
Source of contrast: distribution of a specific protein; differences in amount of elastic light scattering
Visualization methods: EGFP; visualization of contiguous regions
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements

• Cell Image Library Group ID: 10585

• Saccharomyces cerevisiae

• BUB2 protein

• Establishment of protein localization
• GTPase activator activity
• Mitotic spindle orientation checkpoint

• data
• image

• No linguistic content; Not applicable

Samplenumber: 39546

Doi: https://doi.org/10.6075/J0F76BWZ

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL39546

Reference

• Ball DA, Marchand J, Poulet M, Baumann WT, Chen KC, Tyson JJ, Peccoud J. Oscillatory dynamics of cell cycle proteins in single yeast cells analyzed by imaging cytometry. PLoS One. 2011;6(10):e26272. https://doi.org/10.1371/journal.pone.0026272
• PLoS One. 2011;6(10):e26272. Epub 2011 Oct 26. https://www.ncbi.nlm.nih.gov/pubmed/?term=22046265

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2022-08-12