{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1328331600},"Data_type":{"Still_image":false,"Z_stack":true,"Video":false,"Time_series":true},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"39546.zip","Size":1347327112,"Mime_type":"application\/zip"},{"File_type":"Jpeg","File_path":"39546.jpg","Size":23237,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"PUBLISHED":["PLoS One. 2011;6(10):e26272. Epub 2011 Oct 26."],"Contributors":["David Ball","Jean Peccoud"],"PUBMED":["22046265"]},"BIOLOGICALPROCESS":[{"free_text":"mitotic spindle orientation checkpoint"},{"onto_name":"establishment of protein localization","onto_id":"GO:0045184"}],"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"PARAMETERIMAGED":[{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},{"onto_name":"elastic scattering of photons","onto_id":"FBbi:00000587"}],"IMAGEDESCRIPTION":{"free_text":"This is a time lapse image series of Saccharomyces cerevisiae expressing GFP tagged Bub2, a GTPase activator involves in mitotic cell cycle spindle orientation checkpoint and regulation of exit from mitosis.  These phase and GFP z-series images are part of an image group containing CIL 39546-39550.  Note that there are additional groups of images of other cell cycle regulator proteins by the same authors available in the Library."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":{"free_text":"BUB2 protein"},"RELATIONTOINTACTCELL":{"onto_name":"dispersed cells in vitro","onto_id":"FBbi:00000611"},"SOURCEOFCONTRAST":[{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},{"onto_name":"differences in amount of elastic light scattering","onto_id":"FBbi:00000600"}],"GROUP_ID":"10585","MOLECULARFUNCTION":{"onto_name":"GTPase activator activity","onto_id":"GO:0005096"},"TECHNICALDETAILS":{"free_text":"Images were acquired using a 60x 1.42 NA objective lens on a Delta Vision system equipped with a CoolSNAP_HQ2 \/ HQ2-ICX285.  Please read the microscopy section of the referenced paper for details before image analysis."},"DATAQUALIFICATION":{"free_text":"RAW;spatialmeasurements"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":[{"onto_name":"fluorescence microscopy","onto_id":"FBbi:00000246"},{"onto_name":"phase contrast microscopy","onto_id":"FBbi:00000247"}],"DIMENSION":[{"Space":{"Image_size":512,"Pixel_size":{"unit":"nanometers","value":213.4},"axis":"X"}},{"Space":{"Image_size":512,"Pixel_size":{"unit":"nanometers","value":213.4},"axis":"Y"}},{"Time":{"unit":"seconds","value":300,"frame":"61"}},{"Space":{"Image_size":21,"Pixel_size":{"unit":"microns","value":0.5},"axis":"Z"}}],"SPECIESTAXASPECIFIC":{"onto_id":":525"},"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Saccharomyces cerevisiae","onto_id":"NCBITaxon:4932"},"PREPARATION":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"VISUALIZATIONMETHODS":[{"onto_name":"EGFP","onto_id":"FBbi:00000082"},{"onto_name":"visualization of contiguous regions","onto_id":"FBbi:00000396"}]}},"Citation":{"Title":"David Ball, Jean Peccoud (2012) CIL:39546, Saccharomyces cerevisiae. CIL. Dataset","ARK":"ark:\/b7295\/w9cil39546","DOI":"doi:10.7295\/W9CIL39546"}}}