CIL:35428, Mus musculus, oocyte
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- Collection
- Cite This Work
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Brunet, Stephane; Dumont, Julien; Lee, Karen W.; Kinoshita, Kazuhisha; Hikal, Pascale; Gruss, Oliver J.; Maro, Bernard; Verlhac, Marie-Helene (2021). CIL:35428, Mus musculus, oocyte. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0FF3SCJ
- Description
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Time-lapse microscopy of mouse oocytes expressing
YFP-TPX2DN, a truncated version of TPX2 (targeting protein for Xenopus kinesin-like protein, xklp2) which lacks the 118 first
amino acids and injected with a doublestranded siRNA against TPX2, siTPX2-5h. Images were taken every 20 minutes. Times after the resumption of meiotic maturation and germinal vesicle breakdown (GVBD) are indicated in the lower left corner.
Video S8. Found at: doi:10.1371/journal.pone.0003338.s008 - Date Issued
- 2021
- Researchers
- Methods
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Time-lapse images were acquired using a Photometrics CCD camera (CoolSnap HQ2) mounted on a Leica DMI600B microscope using a Leica HC PL APO 20X/0.7 NA objective enclosed in a thermostatic chamber (Life Imaging Services). Immunofluorescence methods can be found in the cited paper and in Brunet S. et al. (1999) J Cell Biol 146: 1–11.
- Technical Details
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Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: Yellow fluorescent proteins from Aequorea
Processing history: unprocessed raw data
Data qualification: Raw - Series
- Scientific Name
- Anatomy
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- Language
- No linguistic content; Not applicable
- Identifier
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Samplenumber: 35428
- Related Resources
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL35428
- Brunet S, Dumont J, Lee KW, Kinoshita K, Hikal P, Gruss OJ, Maro B, Verlhac MH. Meiotic regulation of TPX2 protein levels governs cell cycle progression in mouse oocytes. PLoS One. 2008 Oct 3;3(10):e3338. https://doi.org/10.1371/journal.pone.0003338
- PubMed article: https://www.ncbi.nlm.nih.gov/pubmed/?term=18833336
Source data
Reference
- License
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Creative Commons Attribution 4.0 International Public License
- Rights Holder
- UC Regents
- Copyright
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Under copyright (US)
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12