{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1311739200},"Data_type":{"Still_image":false,"Z_stack":false,"Video":false,"Time_series":true},"CIL":{"Image_files":[{"File_type":"Jpeg","File_path":"35428.jpg","Size":2264,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"35428.zip","Size":3919913,"Mime_type":"application\/zip"},{"File_type":"OME_tif","File_path":"35428.tif","Size":4000000,"Mime_type":"image\/tif"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Href":"http:\/\/www.plosone.org\/article\/info:doi%2F10.1371%2Fjournal.pone.0003338"}],"Contributors":["Stephane Brunet","Julien Dumont","Karen W. Lee","Kazuhisha Kinoshita","Pascale Hikal","Oliver J. Gruss","Bernard Maro","Marie-Helene Verlhac"],"PUBMED":["18833336"]},"BIOLOGICALPROCESS":[{"onto_name":"meiotic spindle organization","onto_id":"GO:0000212"},{"onto_name":"microtubule organizing center organization","onto_id":"GO:0031023"},{"onto_name":"RNA interference","onto_id":"GO:0016246"}],"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Time-lapse microscopy of mouse oocytes expressing\nYFP-TPX2DN, a truncated version of TPX2 (targeting protein for Xenopus kinesin-like protein, xklp2) which lacks the 118 first\namino acids and injected with a doublestranded siRNA against TPX2, siTPX2-5h. Images were taken every 20 minutes. Times after the resumption of meiotic maturation and germinal vesicle breakdown (GVBD) are indicated in the lower left corner.\nVideo S8. Found at: doi:10.1371\/journal.pone.0003338.s008"},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"microtubule","onto_id":"GO:0005874"},{"onto_name":"microtubule organizing center part","onto_id":"GO:0044450"},{"onto_name":"nuclear chromosome","onto_id":"GO:0000228"}],"RELATIONTOINTACTCELL":{"onto_name":"dispersed cells in vitro","onto_id":"FBbi:00000611"},"SOURCEOFCONTRAST":{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},"GROUP_ID":"8819","MOLECULARFUNCTION":{"onto_name":"GTPase activity","onto_id":"GO:0003924"},"TECHNICALDETAILS":{"free_text":"Time-lapse images were acquired using a Photometrics CCD camera (CoolSnap HQ2) mounted on a Leica DMI600B microscope using a Leica HC PL APO 20X\/0.7 NA objective enclosed in a thermostatic chamber (Life Imaging Services). Immunofluorescence methods can be found in the cited paper and in Brunet S. et al. (1999) J Cell Biol 146: 1\u201311."},"DATAQUALIFICATION":{"free_text":"RAW"},"TERMSANDCONDITIONS":{"free_text":"attribution_cc_by"},"IMAGINGMODE":{"onto_name":"confocal microscopy","onto_id":"FBbi:00000251"},"DIMENSION":[{"Space":{"Image_size":190,"axis":"X"}},{"Space":{"Image_size":190,"axis":"Y"}},{"Time":{"unit":"seconds","value":900,"frame":"36"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Mus musculus","onto_id":"NCBITaxon:10090"},"PREPARATION":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"VISUALIZATIONMETHODS":{"onto_name":"Yellow fluorescent proteins from Aequorea","onto_id":"FBbi:00000439"},"CELLTYPE":{"onto_name":"oocyte","onto_id":"CL:0000023"}}},"Citation":{"Title":"Stephane Brunet, Julien Dumont, Karen W. Lee, Kazuhisha Kinoshita, Pascale Hikal, Oliver J. Gruss, Bernard Maro, Marie-Helene Verlhac (2011) CIL:35428, Mus musculus, oocyte. CIL. Dataset","ARK":"ark:\/b7295\/w9cil35428","DOI":"doi:10.7295\/W9CIL35428"}}}