CIL:36637, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist
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- Collection
- Cite This Work
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Allen, Richard (2021). CIL:36637, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0R49PQR
- Description
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Like other cells Paramecium has peroxisomes. These organelles are usually smaller than mitochondria and have a granular appearance and contain a few tubular-like inclusions. These tubules have not been conclusively shown to be continuous with the single limiting membrane of this organelle. In this case the peroxisome has a microtubule lying along its surface. This cell was exposed to HRP for 30 seconds and then chased in HRP-free medium for 3 minutes. The subsequent incubating with hydrogen peroxidase and diaminobenzidine resulted in an electron opaque reaction product in the peroxisomes not because they took in HRP but because the peroxisomes contain large amounts of catalase that results in the reduction of hydrogen peroxidase. HRP can only enter the living cell by endocytosis, phagocytosis, when trichocysts are discharged, at the open cytoproct or when a CV opens. TEM taken on 6/13/79 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 22,500X. Bar = 0.25µm.
- Date Issued
- 2021
- Researcher
- Methods
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This cell was exposed to HRP for 30 seconds and then chased in HRP-free medium for 3 minutes. The subsequent incubating with hydrogen peroxidase and diaminobenzidine resulted in an electron opaque reaction product in the peroxisomes not because they took in HRP but because the peroxisomes contain large amounts of catalase that results in the reduction of hydrogen peroxidase. This was followed by standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
- Technical Details
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Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; lead salt; osmium tetroxide
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed - Scientific Name
- Anatomy
- Topics
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- Language
- No linguistic content; Not applicable
- Identifier
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Samplenumber: 36637
- Related Resource
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL36637
Source data
- License
- Copyright
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Creative Commons Public Domain Dedication
Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Constraint(s) on Use: This work may be used without prior permission.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12