{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1313726400},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"36637.tif","Size":8100000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"36637.jpg","Size":1994460,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"36637.zip","Size":15989102,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"Contributors":["Richard Allen (University of Hawaii)"]},"BIOLOGICALPROCESS":[{"onto_name":"organelle organization","onto_id":"GO:0006996"},{"onto_name":"peroxisome organization","onto_id":"GO:0007031"},{"onto_name":"cytoplasm organization","onto_id":"GO:0007028"}],"PROCESSINGHISTORY":[{"onto_name":"recorded image","onto_id":"FBbi:00000265"},{"onto_name":"film","onto_id":"FBbi:00000303"},{"free_text":"Print from negative scanned for Photoshop."}],"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"Like other cells Paramecium has peroxisomes. These organelles are usually smaller than mitochondria and have a granular appearance and contain a few tubular-like inclusions. These tubules have not been conclusively shown to be continuous with the single limiting membrane of this organelle. In this case the peroxisome has a microtubule lying along its surface. This cell was exposed to HRP for 30 seconds and then chased in HRP-free medium for 3 minutes. The subsequent incubating with hydrogen peroxidase and diaminobenzidine resulted in an electron opaque reaction product in the peroxisomes not because they took in HRP but because the peroxisomes contain large amounts of catalase that results in the reduction of hydrogen peroxidase. HRP can only enter the living cell by endocytosis, phagocytosis, when trichocysts are discharged, at the open cytoproct or when a CV opens. TEM taken on 6\/13\/79 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 22,500X. Bar = 0.25µm."},"ITEMTYPE":[{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},{"onto_name":"illumination by electrons","onto_id":"FBbi:00000273"}],"CELLULARCOMPONENT":[{"onto_name":"peroxisome","onto_id":"GO:0005777"},{"onto_name":"cytoplasm","onto_id":"GO:0005737"}],"RELATIONTOINTACTCELL":{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"},"SOURCEOFCONTRAST":{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},"TECHNICALDETAILS":{"free_text":"This cell was exposed to HRP for 30 seconds and then chased in HRP-free medium for 3 minutes. The subsequent incubating with hydrogen peroxidase and diaminobenzidine resulted in an electron opaque reaction product in the peroxisomes not because they took in HRP but because the peroxisomes contain large amounts of catalase that results in the reduction of hydrogen peroxidase. This was followed by standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http:\/\/www5.pbrc.hawaii.edu\/allen\/)."},"DATAQUALIFICATION":{"free_text":"PROCESSED"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":[{"onto_name":"detection of electrons","onto_id":"FBbi:00000375"},{"onto_name":"film","onto_id":"FBbi:00000303"}],"DIMENSION":[{"Space":{"Image_size":2664,"axis":"X"}},{"Space":{"Image_size":3000,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Paramecium multimicronucleatum","onto_id":"NCBITaxon:44030"},"PREPARATION":[{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},{"onto_name":"osmium tetroxide fixed tissue","onto_id":"FBbi:00000012"},{"onto_name":"tissue in epoxy resin embedment","onto_id":"FBbi:00000018"},{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"}],"VISUALIZATIONMETHODS":[{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},{"onto_name":"uranyl salt","onto_id":"FBbi:00000569"},{"onto_name":"lead salt","onto_id":"FBbi:00000570"},{"onto_name":"osmium tetroxide","onto_id":"FBbi:00000571"}],"CELLTYPE":[{"onto_name":"cell by organism","onto_id":"CL:0000004"},{"onto_name":"eukaryotic cell","onto_id":"CL:0000255"},{"free_text":"Eukaryotic Protist"},{"free_text":"Ciliated Protist"}]}},"Citation":{"Title":"Richard Allen (University of Hawaii) (2011) CIL:36637, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset","ARK":"ark:\/b7295\/w9cil36637","DOI":"doi:10.7295\/W9CIL36637"}}}