Murray, John; Frey, Terence (2021). CIL:41740, Bos sp., heart. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0NS0T8S

Transmission electron micrograph of an unstained, unfixed cytochrome c oxidase crystal prepared from beef heart mitochondria imaged in the frozen hydrated state. A crystalline organization is seen within the ovoid structure in the lower portion of the image.

• 2021

• Frey, Terence
• Murray, John

Samples of cytochrome c oxidase in suspension were applied to holey carbon films in a water saturated atmosphere, blotted, and plunged into a cryogen consisting of a mixture of 95% propane and 5% isopentane cooled by liquid nitrogen. Grids were examined using a Philips 420T TEM and images recorded at 46KX in the frozen hydrated state on Kodak SO-163 film. Negatives were scanned with a pixel size of 20x20 micrometers.
See also: Frey TG and Murray JM 1994. Electron microscopy of cytochrome c oxidase crystals. J Mol Biol 237:275-297.

Preparation: cryofixed tissue
Relation to intact cell: isolated subcellular component
Item type: film
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: intrinsic mass distribution
Visualization methods: visualization of contiguous regions
Data qualification: Raw;spatialmeasurements;intensitiesquantitation

• Cell Image Library Group ID: 12030

• Bos sp.

• Cytochrome c oxidase
• Heart
• Mitochondrion

• Aerobic respiration
• Electron transfer

• data
• image

• No linguistic content; Not applicable

Samplenumber: 41740

Doi: https://doi.org/10.6075/J0NS0T8S

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL41740

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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12