{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1341892800},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"41740.tif","Size":34600000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"41740.jpg","Size":8320953,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"41740.zip","Size":34495250,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"Contributors":["John Murray","Terence Frey"]},"BIOLOGICALPROCESS":{"onto_name":"aerobic respiration","onto_id":"GO:0009060"},"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"Transmission electron micrograph of an unstained, unfixed cytochrome c oxidase crystal prepared from beef heart mitochondria imaged in the frozen hydrated state. A crystalline organization is seen within the ovoid structure in the lower portion of the image."},"ITEMTYPE":{"onto_name":"film","onto_id":"FBbi:00000303"},"CELLULARCOMPONENT":[{"onto_name":"mitochondrion","onto_id":"GO:0005739"},{"free_text":"Cytochrome c oxidase"}],"RELATIONTOINTACTCELL":{"onto_name":"isolated subcellular component","onto_id":"FBbi:00000579"},"SOURCEOFCONTRAST":{"onto_name":"intrinsic mass distribution","onto_id":"FBbi:00000607"},"GROUP_ID":"12030","MOLECULARFUNCTION":{"free_text":"electron transfer"},"TECHNICALDETAILS":{"free_text":"Samples of cytochrome c oxidase in suspension were applied to holey carbon films in a water saturated atmosphere, blotted, and plunged into a cryogen consisting of a mixture of 95% propane and 5% isopentane cooled by liquid nitrogen.  Grids were examined using a Philips 420T TEM and images recorded at 46KX in the frozen hydrated state on Kodak SO-163 film. Negatives were scanned with a pixel size of 20x20 micrometers.\nSee also: Frey TG and Murray JM 1994. Electron microscopy of cytochrome c oxidase crystals. J Mol Biol 237:275-297."},"DATAQUALIFICATION":{"free_text":"RAW;spatialmeasurements;intensitiesquantitation"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},"DIMENSION":[{"Space":{"Image_size":3800,"Pixel_size":{"unit":"nanometers","value":0.43},"axis":"X"}},{"Space":{"Image_size":4538,"Pixel_size":{"unit":"nanometers","value":0.43},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Bos sp.","onto_id":"NCBITaxon:29061"},"PREPARATION":{"onto_name":"cryofixed tissue","onto_id":"FBbi:00000013"},"VISUALIZATIONMETHODS":{"onto_name":"visualization of contiguous regions","onto_id":"FBbi:00000396"},"CELLTYPE":{"free_text":"heart"}}},"Citation":{"Title":"John Murray, Terence Frey (2012) CIL:41740, Bos sp., heart. CIL. Dataset","ARK":"ark:\/b7295\/w9cil41740","DOI":"doi:10.7295\/W9CIL41740"}}}