CIL:39503, Vorticella microstoma, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist
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- Cite This Work
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Allen, Richard (2021). CIL:39503, Vorticella microstoma, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J02F7MRH
- Description
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Freeze fracture of Vorticella microstoma. This cell had been placed in a relaxing medium of saturated sodium pyrophosphate plus 0.01M EDTA (Stain Tech. 47:37, 1972) for 3 hours. A myoneme enclosed by ER membrane is fractured at the cell’s periphery. In this cell it appears that the ER wraps around the myoneme. The P-fracture face of the plasma membrane is studded with many IMPs of significant size in contrast to the E-face of the outer alveolar membrane which has few IMPs. Bits of the P-face of the inner alveolar membrane
show many IMPs on that face also. The P-face of the cytosolic-touching membrane of the ER and the E-face of the myonemal-touching membrane of the ER are visible. Linear arrays of IMPs on the E-face next to the myoneme may be transmembrane proteins associated with the linkage complex. Mitochondria are the most prominent organelles in the cytosol and their tubular cristae sometimes show signs of decoration with F1F0 ATP synthases. large arrow, direction of shadowing of freeze fracture replica. TEM taken on 11/11/75 by R. Allen with JEM 100B operating at 80kV. Neg. 11,015X. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/). - Date Issued
- 2021
- Researcher
- Technical Details
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Preparation: freeze_fracture/freeze_etch
Relation to intact cell: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: electron density
Parameter imaged: elastic scattering of electrons; electron density; elevation
Source of contrast: differences in deposition of metal shadow
Visualization methods: transmission electron microscopy (TEM)
Processing history: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film
Data qualification: Raw;spatialmeasurements;intensitiesquantitation - Series
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- Identifier
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Samplenumber: 39503
- Related Resource
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL39503
Source data
- License
- Copyright
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Creative Commons Public Domain Dedication
Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Constraint(s) on Use: This work may be used without prior permission.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12